[Histonet] weils stain for myelin

[Tony Henwood]

Weil's Hx is made up similarly to Verhoeff's Elastic Stain Hx. Staining
with Verhoeff's is clearer and easier to differentiate if aged alcoholic
Hx is used. If you have any alcoholic HX that has been prepared as a
stock for another technique, you can use it in the Weil's method. I
dilute it with ethanol to the required concentration (we have two stock
10% Hx in ethanol solutions available and as one is used, the other has
ripened and is ready for use).

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of stevenk
Sent: Wednesday, 29 November 2006 3:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] weils stain for myelin



	I am asking for a colleague who was doing a Weil's stain for
myelin.

	One of the ingredients, 2% alcoholic haematoxylin requires a 
ripening process for at least 6 months.

	As the student cannot wait that long, I was wondering if 
anyone could supply a quicker method, or suggest something to hasten 
the ripening process of the above.

	Thanks from down under

	Stephen
-- 
|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Australia
|Ph: + 61 2 9351 6143
|Fax:+61 2 9351 3429

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