[Histonet] Repost: Question on calcified samples

Gayle Callis gcallis <@t> montana.edu
Tue Nov 28 10:22:12 CST 2006

Instrumedics Cryojane Tape Transfer, it will work in most cryostats that 
have a temperature range down to -35C, and D profile tungsten carbide 
cryostat knives (there require a universal knife holder in the cryostat) 
Delaware Diamond Knives sell these knives, they cost around $1200 per knife 
and then ~$250 to recondition.  The Cryojane runs ~$7000 or more (the price 
may have gone up).

Coral will be very hard to work with, and doing frozen sections may not be 
ideal.  It is not like bone as pointed out in a previous email.

At 06:38 AM 11/28/2006, you wrote:
>   I am a histotechnology student, and my classmates and I will be working 
> with some coral samples this year. I was just wondering if there was a 
> way to prepare frozen sections on calcified samples, and if so, what 
> equipment is needed?
>   Thank you,
>   Adrienne Mazzante
>   Histotechnology Program
>   Medical University of South Carolina
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>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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