[Histonet] automated immuno stainer

[Patti Loykasek]

Hi Christine. I believe that a good, well trained tech can achieve good
immunos on any type of platform. I think the main issues are your
preferences: do you want an open or closed system - any detection system or
only the instrument company, concentrated or prediluted antibodies,etc?
Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease
of waste disposal? Can you use the clones of antibodies you have currently
validated on the instrument? User friendly? Those are the main issues I can
think of at the moment - my brain is still tired from all the turkey
tryptophan!  
Most companies are more than happy to provide you with a demo & they may
even help work up some things for you.
As for my preference - it is Dako. We have several of the 48 slide
configured Autostainers. I'd be happy to respond to any questions you might
have about the Dako.
Good luck in your stainer hunting.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA







> Hello all! I hope everyone enjoyed their breif repreive from stress. I was
> wondering if anyone could give me some input on automated stainers for
> immunohistochemistry. Currently we hand stain and our volumes are steadily
> increasing. We are looking at automation as we probably do up to 12-15 stains
> per day and plan on starting ER and PR. I heard Vantana is very good. How
> about Leica?  (Theirs does both immunos and ordianry special stains.) Anyone
> have any experience they would like to share? We are an Ascension Health
> affiliated hospital as that may matter in the negotiations. Thanks for any and
> all opinions.
> Respectfullly,
> Christine Tambasco, HT (ASCP)
> St. Mary's Hospital
> Amsterdam, New York 12010
> 518-841-7287
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