[Histonet] OCT questions

[Sebree Linda A.]

In a previous job, I used 5% gum tragacanth to freeze rabbit myocardium
in.  It cut well and orientation was quite easy as it is stiffer than
OCT.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Surita
Banwait
Sent: Wednesday, November 22, 2006 12:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] OCT questions



 

Hi There,

Would anyone happen to know if there is a way to attach frozen tissue to
cryostat chucks without using OCT media or super glue?  

 

Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media?

 

Thanks,

Surita


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