[Histonet] Normal Controls for Cerb-B2

[Tony Henwood]

Bryan,

You are absolutely right. 
This raises the question of thresholds. 
At what dilution of antibody should staining reflect over-expression?
How many labs would call a borderline over-expression positive? 
This would depend on the dilution of the Cerb-B2 antibody as well as the
sensitivity of the detection system.
I think I will need to peruse the external QAP figures to see if this is
an issue.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Bryan Hewlett [mailto:bhewlett <@t> cogeco.ca] 
Sent: Tuesday, 21 November 2006 1:10 AM
To: Tony Henwood; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Normal Controls for Cerb-B2


Tony,

The vast majority of normal epithelial cells express HER2 protein on
their 
surface.
However, you would not want to use these as a procedural control, since
the objective is to demonstrate OVER-EXPRESSION of this normal 
protein.
Detection of normal levels of HER2 would invalidate the test.

Bryan

----- Original Message ----- 
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 20, 2006 12:15 AM
Subject: [Histonet] Normal Controls for Cerb-B2


Hi all,
Apart from Breast and Ovarian tumours (which are rare in pediatric
pathology), does anyone know what normal tissue elements contain this
protein that can be demonstrated using immunohistochemistry?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist The Children's Hospital at Westmead, Locked
Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




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