[Histonet] RE: Alizarin red S - Calcium

[Jackson, Johanna]

The pH or Alizarin Red is very critical so I would try and keep it as close to the recommended pH as possible.  Also, be careful when you wash the section/cells with PBS or something as this really mucks it up... may be obvious but I found out the hard way!!
 
Good luck!
 
Jo
 
 
Stem Cell Imaging
MRC Clinical Sciences Centre
Imperial College London
Hammersmith Hospital Campus
Du Cane Road
London
W12 0NN
<mailto:johanna.jackson <@t> csc.mrc.ac.uk>  


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Message: 6
Date: Mon, 20 Nov 2006 10:14:31 -0500
From: Diego Villegas <dfvilleg <@t> mtu.edu>
Subject: [Histonet] Alizarin red S - Calcium
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4561C657.1030005 <@t> mtu.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi all,

The protocol for Alizarin red S to identify calcium suggest a solution
pH 4.1 to 4.3.  The Alizarin solution of our provider has a 4.5 to 5
pH.  Could I use it, or is critical the pH?

Diego
Biomechanics Lab
Michigan Technological University.