[Histonet] RE: Alizarin red S - Calcium
johanna.jackson <@t> csc.mrc.ac.uk
Mon Nov 20 13:23:48 CST 2006
The pH or Alizarin Red is very critical so I would try and keep it as close to the recommended pH as possible. Also, be careful when you wash the section/cells with PBS or something as this really mucks it up... may be obvious but I found out the hard way!!
Stem Cell Imaging
MRC Clinical Sciences Centre
Imperial College London
Hammersmith Hospital Campus
Du Cane Road
<mailto:johanna.jackson <@t> csc.mrc.ac.uk>
Date: Mon, 20 Nov 2006 10:14:31 -0500
From: Diego Villegas <dfvilleg <@t> mtu.edu>
Subject: [Histonet] Alizarin red S - Calcium
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4561C657.1030005 <@t> mtu.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
The protocol for Alizarin red S to identify calcium suggest a solution
pH 4.1 to 4.3. The Alizarin solution of our provider has a 4.5 to 5
pH. Could I use it, or is critical the pH?
Michigan Technological University.
More information about the Histonet