[Histonet] Low Yield FNA samples

[sheila adey]

Hi
We also were experiencing the same situation. We use to use the Thermo 
Shandon cysto spin chamber but we found that some of the material would 
still be in the funnel. So... actually by mistake one day I noticed that 
their 2 reagents sent with the pack, one is a formalin fixative and the 
other is a Calcium Chloride; form a nice clot.
Now we just add 4 drops of the Blue reagent to the cell button and 
resuspend. Then add at least 5 drops of the Clear reagent to form a great 
clot. Then pull it out and wrap it in tissue paper.We have really increased 
the retrieval of cells
Hope this helps





>From: "Patterson, Patricia" <PatPatterson <@t> mhd.com>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Low Yield FNA samples
>Date: Fri, 17 Nov 2006 17:13:49 -0600
>
>
>Greetings Histonetters!
>
>We have some very low yield FNA's here and we're trying to find a better
>way to handle them to optimize what our Histology lab needs to deal
>with.
>
>
>
>Currently we do a simple spin down with 95% and create a cell block.
>But sometimes the amount we pull from tube is very small.
>
>We've tried a Fibrin clot method using plasma or thrombin - but our
>method seems time consuming.
>
>
>
>Someone mentioned an agar cell method or a colloidal bag method but we
>don't know anyone who is doing them.
>
>Any suggestions would be greatly appreciated.
>
>
>
>Thanks
>
>Pat Patterson
>
>Methodist Dallas Medical Center
>
>patpatterson <@t> mhd.com
>
>P - 214-947-3538
>
>F - 214-947-3524
>
>
>
>
>
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