[Histonet] black wax in dissecting pans

[Jo Dee Fish]

Liz,
I make mine "homemade" starting with 1 lb of pink dental wax (a bit softer
than paraffin so it won't split or crack when a pin is stuck in it) and
black wax dye, I use about 1/4 oz. per lb. of wax.  I use it for exactly
what you are doing, pinning out aortas.  
Good luck,
Jo Dee


Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Friday, November 17, 2006 1:02 PM
To: 'Bonnie Whitaker'; 'Histonet'
Subject: RE: [Histonet] black wax in dissecting pans

We thought about that but we are using the wax for pinning "en face"
analysis of mouse aorta's and we thought that candle wax would be to brittle
and we were uncertain if we could get the black candle wax dark enough for
imaging.  We are using special pins that we get through fine since tools
they are 0.1mm in diameter.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540
liz <@t> premierlab.com www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: Bonnie Whitaker [mailto:bwhitaker <@t> brownpathology.com]
Sent: Friday, November 17, 2006 1:47 PM
To: 'Liz Chlipala'; 'Histonet'
Subject: RE: [Histonet] black wax in dissecting pans

Bennie & Smith perhaps : )

No, seriously, get some of the coloring agent from a candle-making
supply/craft store.  I'd bet that would work to color regular paraffin.

Bonnie Whitaker


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Friday, November 17, 2006 2:02 PM
To: 'Histonet'
Subject: [Histonet] black wax in dissecting pans


Hello All

 

Does anyone know were I can get the black wax that is in used in the metal
dissecting pans.  I know that I can order the pans with the wax, but I was
wondering if anyone out there knows if I can just purchase the wax alone.

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

P.O. Box 18592

Boulder, CO 80308

phone (303) 735-5001

fax (303) 735-3540

liz <@t> premierlab.com

www.premierlab.com

 

Ship to Address:

 

Premier Laboratory, LLC

University of Colorado at Boulder

MCDB, Room A3B40

Boulder, CO 80309

 

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