[Histonet] calcium in the enthesis ligament

[Diego Villegas]

Hi all,

I am working with bovine ligaments, and I  have to identify the calcium 
present on the knee ligament enthesis. I would like to know if Alizarin 
red S is the best to this and, the protocol suggest a pH solution of 4.1 
- 4.3.  The stain solution of our supplier has a pH ranged between 4.5 
to 5.  Could I work with this stain solution?.

Thanks,

Diego Villegas
Biomechanics Lab
Michigan Tech Uninversity