[Histonet] freezing brain slices

[Stacey Barrick]

Hello..
   
  I'm trying to freeze brain slices for immuno. The slices are 250uM cut with a vibratome. I have been trying to embed these slices in OCT so that I may section them using the cryostat. It seems as though the tissue is not freezing flat. Does anyone have any suggestions? I cannot get the slices to freeze flat no matter what i try. 
   
  Thank you!
  Stacey 
  barrickstacey <@t> yahoo.com
   

 
---------------------------------
Sponsored Link

$420,000 Mortgage for $1,399/month -   Think You Pay Too Much For Your Mortgage? Find Out!