[Histonet] tissue-tek mega cassettes

Bobrowitz, Carol carolb <@t> phys.mcw.edu
Wed Nov 15 14:10:23 CST 2006



I've been purchasing from Sakura the tissue-tek mega cassettes.

I use the HM355S Microm microtome.

These cassettes were the only ones I could find to purchase that fit the microtome chuck.

I tested several other makes all were loose fitting and caused sectioning problems.


Sakura has changed the design of their tissue-tek mega cassettes.

They no longer fit snuggly in the chuck.  If you put the block in horizontally or vertically 

it does not fit correctly.


Has anyone else had this problem?


If I use a piece of small cardboard, like the old days, it wouldn't work.  

The vertical measurement is too short for a snug fit.


I need a mega cassette that fits snuggly in the chuck.  Does anyone purchase these 

without this problem?


May I please have the name of your source?


Any vendor who sell mega cassettes 1 ½ " by 1" and ½" deep  please send me some 

samples to test.


Any help would be appreciated. 


Thank you,


Carol Ann Bobrowitz

Medical College of Wisconsin

Department of Physiology

MEB RM #4940

8701 Watertown Plank Road

Milwaukee, Wisconsin   53226


cbobrowi <@t> mcw.edu

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