[Histonet] ATTN: Veterinary Diagnostic Laboratories, Revisiting Bovine viral diarrhea antibody, BVD

[Gayle Callis]

Dear All,

Our veterinary diagnostic laboratory is looking for more clones 
(monoclonal) or  a polyclonal BVD antibody which is consistent and 
reliable.  In doing a Histonet Archive search, it seems some labs have 
experienced staining problems and they were looking for the antibody also.

They need both unconjugated and a FITC conjugate.  It has been a long time 
since I had to work with antiBVD but did have success with  mouse anti BVD, 
clone 15c.5 from Cornell.  It has been a long time since we purchased this 
and I am not sure you buy it from Cornell anymore??   One polyclonal they 
are using now for cell cultures is a swine antiBVD-FITC.   Any suggestions 
for other antibodies out there will be welcomed.  I would like to know what 
kits laboratories prefer to use for enzyme immunohistochemistry along with 
a favored enzyme digestion on FFPE tissues with this antibody.  For 
research purposes, my lab did not use kits and never had any staining 
problems but the diagnostic lab wants the convenience of using kits and AEC 
chromogen.   Thanks in advance



Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)