[Histonet] Re: Helicobacter controls
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Nov 11 13:42:52 CST 2006
I used modified Steiner for H. pyloris and this is an unspecific silver stain, it will stain bacteria so, after much arguments with the pathologists, I was allowed to use the organisms in appendix sections as + controls, because if those bacteria were stained, so would H. pyloris. That made my work much easy!
RSRICHMOND <@t> aol.com wrote:
Lee and Peggy Wenk asked about Helicobacter stain controls.
Every lab I've ever worked in just used its positive slides as controls, and
I've found that to be satisfactory. The toluidine blue (e.g. Diff-Quik) stains
don't really need controls. In labs where the histotechs never look at a
slide, it's common to find that the control isn't positive - I sign these out as
"with a suitable control" for regulatory purposes. - The IHC of course requires
Rather rarely these days, you get a gastrectomy specimen that's positive, and
one of these would give you controls for the rest of your life - look for
Helicobacter heilmanii (AKA Gastrospirillum hominis) has the same IHC
reactivity as H. pylori - there's literature about this - it's more common in Japan
than in the USA.
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