[Histonet] Autofluorescence

Stephen Clark sclark59 <@t> hotmail.com
Thu Nov 9 09:43:14 CST 2006

My name is Stephen Clark and i work in the neuro lab at Eastern Illinois University.  We use fluorescent immunohistochemistry to stain the Olfactory Epithelium of mice and have recently had a problem with autofluorescence of our tissues.  They are fixed in 4% paraformaldehyde in PBS and i have already tried sodium borohydrate with little success.  I have read about photobleaching using UV, neon, and lights of specific wavelengths (488 and 633nm).  I am just wondering if anyone utilizes photobleaching via a light source and where it would be possible to purchase them.  I am also wondering if the 18um sections might be a little too thick and whether that would have an increased affect on autofluorescence.  Any tips would be appreciated.

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