[Histonet] processing polyester filters

[Perry, Margaret]

I am resending this message because we did not receive Vol 36 Issue 6 of
the digest.  We have a researcher who is growing porcine intestinal
epithelial cells on snapwell polyester filters.  He wants us to stain
with the PAS and alcian blue.  Our first attempt did not work.  The
filters were fixed in 4% paraformeldehyde for 10 minutes and processed
in our regular overnight processing schedule. Our first thought is that
we processed way too long.  What type of processing schedule should we
be using and how long should we be fixing these filters?  

 

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007