[Histonet] processing poyester filters

[Andrea Grantham]

One of my past projects involved processing and sectioning cells grown on 
corning transwell inserts. Could they be similar to the filters that you 
are talking about?
For these inserts, I processed using our old processor - an old Tissue Tek 
dip n'dunker for one hour in each station including 2 paraffins (our 
regular overnite processing schedule). They cut and stained great.
I didn't fix them myself but the inserts were flooded with formalin - 10% 
(as recommended by corning) and allowed to fix for one hour at RT. After 
fixation they replaced the formalin with 70% ETOH and brought them here to 
the lab for processing.

Andi Grantham



At 03:22 PM 11/7/2006 -0600, you wrote:
>We have a researcher who is growing porcine intestinal epithelial cells
>on snapwell polyester filters.  He wants us to stain with the PAS and
>alcian blue.  Our first attempt did not work.  The filters were fixed in
>4% paraformeldehyde for 10 minutes and processed in our regular
>overnight processing schedule. Our first thought is that we processed
>way too long.  What type of processing schedule should we be using and
>how long should we be fixing these filters?
>
>
>
>Margaret Perry HT (ASCP)
>
>IHC Lab Manager Veterinary Science
>
>Animal Disease Research and Diagnostic Lab
>
>South Dakota State University
>
>Box 2175 North Campus Drive
>
>Brookings SD 57007
>
>
>
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.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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