[Histonet] processing poyester filters

[Perry, Margaret]

We have a researcher who is growing porcine intestinal epithelial cells
on snapwell polyester filters.  He wants us to stain with the PAS and
alcian blue.  Our first attempt did not work.  The filters were fixed in
4% paraformeldehyde for 10 minutes and processed in our regular
overnight processing schedule. Our first thought is that we processed
way too long.  What type of processing schedule should we be using and
how long should we be fixing these filters?  

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007