[Histonet] Fwd: Bubble artifact for Histonet

[LINDA MARGRAF]

Histonetters:
We are looking for help in identifying a particular artifact that we have seen off and on for the last few years.  We call it "bubbling" artifact.  The artifact seems to be somewhat random as shown in the pictures provided by the link
http://www.histonet.org/site_images_frame.asp 

(Called bubbles and no bubbles posted by Linda Margraf)
(If link doesn't work go to www.Histonet.org and click on "view list serv images"...these pictures are at the top)

 In one picture, the artifact is clearly visible; in another picture, it is not present at all.  These pictures are of a particular GI biopsy and the sections are right next to each other on the slide.  Same case, same ribbon, same biopsy, same staining, same processing, same coverslipping...everything is exactly the same except that one has the artifact and the other does not.  Has anybody else ever encountered something like this?  And if so, were you able to narrow down the cause to something specific?  Any help you can offer us would be greatly appreciated
Thanks!
Michelle L McNeese, HT (ASCP)
Lead Tech, Histology
Department of Pathology
Childrens Medical Center
(214) 456-6146
Email:  michelle.mcneese <@t> childrens.com