[Histonet] Frozen section problems!!
Adam M Gomez
amgomez <@t> mail.unomaha.edu
Mon Nov 6 13:14:13 CST 2006
We are wanting to section peripheral gang cryostat. Our current procedure is as follows:
1)perfused with PBS/8% Para, ganglia tissue removed
< color 10 min.
3)placed in 30% sucrose overnight (or until 4)rinsed in PBS/dH20
5)embedded in OCT
6)frozen rapidly in liquid nitrogen
7)tissue allowed to equilibrate to cryostat temp & sectio cryostat (-18 C)
9)Slides are dipped in clearing agent (xylenes) alcohol (95%, 70%, 50%), dH2O, cresyl violet stain, dH2 95%, & xylenes...and coverslipped immediately.
The problem we are having is that the tissue looks outstanding after sectioning as long as it remains wet (i.e., when we take a look at
tissue d we're losing ce seems that the dehydr tissue is the culprit. We have tr procedure but have yet to find a successf the tissue. If you have any suggestions p problem has been detrimental to our work for the pa
Research&nb University of Nebraska at Om Department of Psychology
1. 3D"mailto:amgomez <@t> mail.unomaha.edu"
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