[Histonet] Re:Formalin Fixation & IHC
carl.hobbs <@t> kcl.ac.uk
Thu Nov 2 13:57:42 CST 2006
For paraffin wax sections requiring Heat-induced antigen
retrieval/unmasking, it is not often that one reads such pragmatically
accurate, apposite and accessible statements/advice such as
My only problem with it is.......
I wish I could have stated it so well!
The Researchers who carry out Immuno on "briefly" Formalin-fixed
cryo/vibratome tissues/sections and do not need any Ag retrieval/unmasking
to get good results should also gain knowledge from Brian's words of wisdom.
For them, I would add that when you get a poor result in such tissue prep.
sytems, try pwax!
An example of many such situations: In my Research Centre, a PhD
student has struggled to get good results for astrocyte subset
localisation, using "PFA"-fixed, perfused cryo/vibratome sections with anti
NG2. This went on for a year and then they came to me, for advice.
Once we fixed her specimens in good ol' "10% Formalin" for a week,
pwax-processed them and carried out Immuno-DAB/Fluorochrome on the AR
A year wasted!!
"Sure, "horses for courses"..
Great respect, Brian.
If I may, I will add the guts of your post to my SOPs.
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