[Histonet] pH of EDTA

[donna rossi]

   We are having a problem with EDTA pH 8.0 from LabVision.  When we take
   the pH of the concentrate it is 8.O.  When we dilute it as recommended
   it  changes  to  6.0.   Lab  Vision  replaced our bottles  but the new
   bottles  also  come  out  to  6.0 when diluted.  We took the pH of the
   Distilled  water here and got 7.1.  What could be the problem.?  Do we
   adjust  the distilled water to a higher pH?  How do we do that?  Do we
   then  need to validate all of our antibodies that use EDTA  using the"
   new"  EDTA?  We have  also had this problem with Zymed EDTA but not as
   radical  a  difference as  the  Labvision.   It  was suggested that we
   switch  to  ready-made  EDTA but we have a quantity of this and do not
   have  the room to store multiple big bottles of this retrieval buffer.
   Can someone give me a suggestion?  Thanks,  Donna, SRHS
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   [1]Use your PC to make calls at very low rates 

References

   1. http://g.msn.com/8HMAENUS/2749??PS=47575