[Histonet] TRAP staining on undecalcified bone

Tamara Franz-Odendaal Tamara.Franz-Odendaal <@t> msvu.ca
Wed Nov 1 09:55:33 CST 2006


We have done TRAP staining in our lab on undecalcified bone. Refer to
this paper for a great protocol.
Witten, PE., Hansen A., Hall, BK.  Journal of Morphology 250: 197-207.


Tamara Franz-Odendaal (PhD)
Biology Dept, 
Mount Saint Vincent University
166 Bedford Highway
Halifax, NS,  B3M 2J6

Tel: 902 - 457 6140
Fax: 902 - 457 6455

>>> "Tiffany Pitts" <tes4 <@t> u.washington.edu> 10/31/2006 6:53 PM >>>
Hello all,
I am trying to do TRAP staining on undecalcified mouse bones cut from 
Methylmethacrylate embedded blocks. My problem is that I cannot seem to
a protocol that works well and/or is on the market any more. Does
know of a protocol I can use or (even better) a kit? Our in-house
is light on details and I'm not sure if I'm doing it right.
Thank you

Tiffany Pitts
Department of Urology
University of Washington
Seattle, WA 

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