[Histonet] GAD vs. paraformaldehyde

[john <@t> greenhorn.org]

On 31 May 2006, at 19:12, x j wrote:

> I read an article on  
> http://www.histosearch.com/histonet/May00A/ 
> Re.GABBAandalcoholfix.html). It is very informative.
>
> I am doing GAD 65/67 experments. I have the following questions:
>
> 1). on the webpage  
> http://www.histosearch.com/histonet/Aug02A/RE.4paraformaldehyde.html,  
> it is said "It suggests that the solution was fresh and that very  
> little formic acid or methanol had had time to form". If old  
> paraformaldehyde solution is used, does the formic acid or methanol  
> have negative effects on the later GAD staining?
>
> 2). Why people use alchol for perfusion and post fix ("profusing with  
> 80% alcohol", "I drop mouse
> brains into fresh 80% (alcohol)... to post fix" in  
> (http://www.histosearch.com/histonet/May00A/ 
> Re.GABBAandalcoholfix.html). It is very informative)
>
> Thank you very much!
> Jun
>
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