[Histonet] Re: Meiosis and Mitosis
Laurie Reilly
laurie.reilly <@t> jcu.edu.au
Tue May 30 18:33:40 CDT 2006
Dear All,
Mitosis in mammalian cells is well demonstrated in the granulosa cell layer
surrounding ovarian follicles. Pig ovary is good because they have many
developing follicles at one time.
These mitotic figures appear more like what is seen in neoplastic changes
than do the mitotic figures seen in onion root tips, which always seem
artificially clear.
Regards, Laurie.
At 01:24 PM 30/05/2006 -0700, Jen, Shirley wrote:
>Hi Lina,
>My experience is that you will find a good cell meisis (spermatogenesis)
>in Bouin's fixed paraffin section of testes and you will find some mitosis
>cell division in formalin fixed intestinal sections.
>Good Luck.
>Shirley Jen
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>histonet-request <@t> lists.utsouthwestern.edu
>Sent: Tuesday, May 30, 2006 10:11 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 30, Issue 44
>
>
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>Today's Topics:
>
> 1. anti-Abeta antibodies (Dumont, Nancy)
> 2. RE: Methods for meiosis and mitosis (Rittman, Barry R)
> 3. RE: To Ventana or not to Ventana (Deltour, Douglas D. (HM2))
> 4. Ventana Benchmark (Sanders, Julie, VHACIN)
> 5. Ventana Benchmark XT (Malam Jacqueline)
> 6. question (gayle brosnanwatters)
> 7. RE: whole villi (Jackie M O'Connor)
> 8. Re: Ventana Benchmark (Jackie M O'Connor)
> 9. Energy Beam (Rita Riddle)
> 10. RE: Energy Beam (Weems, Joyce)
> 11. RE: Ventant Benchmark (Sebree Linda A.)
> 12. Cre, eGFP, and B-Gal Antibodies (Rogers, Rhonda)
> 13. slow draining paraffin dispenser (Walters, Katherine S)
> 14. Re: [Histonet]Hospital costs/new topic (Geoff McAuliffe)
> 15. RE: slow draining paraffin dispenser
> (Favara, Cynthia (NIH/NIAID) [E])
> 16. RE: slow draining paraffin dispenser (Bonner, Janet)
> 17. floaters (Webb, Dorothy L)
> 18. Stent paper and Histonet connection (black)
> 19. Re: methyl green counterstain (Osborn, Barbara)
> 20. RE: Ventana Benchmark closed systems vs Open system st ainers
> - a diatribe (Morken, Tim - Labvision)
> 21. Re: floaters (Rene J Buesa)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Mon, 29 May 2006 16:23:13 -0400
>From: "Dumont, Nancy" <ndumont <@t> neurochem.com>
>Subject: [Histonet] anti-Abeta antibodies
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>
><1FAA5B74210C9A45A37A46EF6A969761076446 <@t> svrneurochem9.neurochem.local>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Hello,
>
>I am looking for information on antibody affinity against Abeta peptide,
>at either the N-terminal part (such as 6E10 monoclonal antibody) or C
>terminal (such as polyclonal antibodies specific for Aß40 or Aß42 from
>Chemicon). Does anybody have some data sources to mention to me?
>
>I also have a question about pan-Abeta antibody. We constantly have
>cellular labeling with the pan-Abeta antibody from BioSource (sequence 15
>to 30) when we process brain sections of transgenic mice. Does someone
>know if this antibody also labels APP? In fact, it seems that the
>labeling is indeed nuclear. Controls without primary or secondary
>antibodies are totally blank. A reference cited by the company also says
>that preabsorption of the antibody with the peptide leaded to the absence
>of any labeling.
>
>Thanks in advance for any advice!
>
>
>Nancy Dumont
>Research technician
>Neurochem Inc.
>Montreal, Canada
>L'information contenue dans ce courriel est confidentielle et protégée par
>le secret professionnel. Elle n'est destinée qu'à l'usage du destinataire
>indiqué ci-dessus. Il est strictement interdit de distribuer ce document
>ou l'information qu'il contient. Si vous avez reçu ce courriel par erreur,
>ou s'il ne vous est pas destiné, veuillez le mentionner immédiatement à
>l'expéditeur et détruire tous les exemplaires de ce courriel. Merci. /
>
>This email is intended only for the party to whom it is addressed, and may
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>
>------------------------------
>
>Message: 2
>Date: Mon, 29 May 2006 18:51:30 -0500
>From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
>Subject: RE: [Histonet] Methods for meiosis and mitosis
>To: "Lina Vieira" <livieira <@t> ualg.pt>, "Histonet"
> <histonet <@t> pathology.swmed.edu>
>Message-ID:
> <EA1FDD2A141B7448B4B1AFFFCAC08DE406169C14 <@t> UTHEVS1.mail.uthouston.edu>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Lina
>One of the simplest for mitosis is to use onion root tips, they grow
>rapidly and can process them via easilty. For meiosis can use mouse
>testis. Chromosomes easily seen using either Feulgen's reaction or iron
>hematoxylin. Barry
>
>________________________________
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Lina Vieira
>Sent: Mon 5/29/2006 11:17 AM
>To: Histonet
>Subject: [Histonet] Methods for meiosis and mitosis
>
>
>
>Hi,
>I need to make some slides wich provide a good image of mitosis and
>meiosis for education purposes and I have only week to do it! Have You any
>suggestions? Protocols? Bibliography? Tanks in advance,
>
>Lina Vieira
>University of Algarve
>Portugal
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>------------------------------
>
>Message: 3
>Date: Tue, 30 May 2006 06:19:32 -0400
>From: "Deltour, Douglas D. (HM2)" <DDDeltour <@t> mar.med.navy.mil>
>Subject: RE: [Histonet] To Ventana or not to Ventana
>To: "'Scott A. Ely'" <sae2001 <@t> med.cornell.edu>,
> histonet <@t> lists.utsouthwestern.edu
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Message-ID:
> <3F500F8B416C554EBB21FF16642F72E959CE9F <@t> marxchg03.mar.med.navy.mil>
>Content-Type: text/plain
>
>I use two of the Benchmark XT's and love them. You could use the other
>open systems and cut down on cost per slide. You have to take into
>consideration your staffing. You can train other techs easily on the
>Benchmark. It would be much easier to rotate staff or make up for those
>sick days with the XT. If you tried to train them on an open system which
>includes making up buffers and so on, then it may be more of a headache.
>Good luck.
>
>Douglas Deltour HT(ASCP)
>
>-----Original Message-----
>From: Scott A. Ely [mailto:sae2001 <@t> med.cornell.edu]
>Sent: Friday, May 26, 2006 12:59 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Ventant Benchmark
>
>We are considering buying some new equipment. I would like to know who
>uses the Ventana Benchmark
>immunostainer or other immunostainers and what you like and don't like
>about it.
>
>Scott Ely, MD MPH
>Section of Hematopathology
>Department of Pathology
>Weill Medical College of Cornell University
>New York Presbyterian Hospital
>525 E. 68th Street
>New York, NY 10021
>PH: 212-746-2442
>
>Legal Confidentiality Notice: This e-mail message, including any
>attachments, is for the sole use of the original
>intended recipient(s) selected by Dr. Ely and may contain confidential and
>privileged information. Any
>unauthorized review, use, disclosure or distribution is prohibited. If you
>are not the recipient specified by Dr.
>Ely, please contact the sender by reply e-mail and destroy all copies of
>the original message.
>
>----- Original Message -----
>From: histonet-request <@t> lists.utsouthwestern.edu
>Date: Friday, May 26, 2006 12:29 pm
>Subject: Histonet Digest, Vol 30, Issue 39
>
>
>
>------------------------------
>
>Message: 4
>Date: Tue, 30 May 2006 07:06:39 -0400
>From: "Sanders, Julie, VHACIN" <Julie.Sanders <@t> va.gov>
>Subject: [Histonet] Ventana Benchmark
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> <2D4ACE41DEFE93428F23D77988EFBCB50E5FEA <@t> VHAV10MSGA2.v10.med.va.gov>
>Content-Type: text/plain; charset="iso-8859-1"
>
>I agree with Joe, the cost of running the Ventana is high. My chief is
>always trying to figure out a way for us to not do immunos in house just
>because of this. With that said, our Ventana sales rep has given us the
>best prices possible for the volume we run, which is not that high
>compared to large hospitals/labs. I wish there were some way to keep the
>cost down for running this particular machine (we have a Benchmark XT),
>but there isn't and costs can only go up, as they do every year. However,
>we like Ventana, the technology, tech support, and sales support. Our
>pathologists would walk if we ever stopped using it as it has provided
>them with the best, most reliable technology we have found on the market.
>
>"How come every one talks about the reliability and walk away technology, but
>no one ever talks about the price of running these machines? Shouldn't cost
>be a factor also?
>I've been in budget meetings all week. The only thing I heard was how
>expensive it is to run immunos."
>
>Julie Sanders, BA, HT(ASCP)
>Supervisor, Anatomic Pathology
>Cincinnati VAMC
>
>
>
>------------------------------
>
>Message: 5
>Date: Tue, 30 May 2006 12:12:37 +0100
>From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
>Subject: [Histonet] Ventana Benchmark XT
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <F2D584187C727B4CBFE008435881E8970D0AC0 <@t> rlixch>
>Content-Type: text/plain
>
>We have used a Benchmark XT for two and a half years now and it's been great.
>Advantages
>- complete standardisation
>- a noticeable saving in time as the antigen retrieval is automatic
>- 3 primary antisera incubation temperatures (it is interesting to
>discover that some like it at room temp and some like it hot!)
>- the variable antigen retrievals, primary incubation times and
>temperatures give plenty of choices for optimisation
>- making antisera up in "bulk" in prep kit dispensers saves you having to
>make them up each time with some waste and, with some antisera working at
>higher dilutions, this can lead to savings
>- prompt servicing, excellent help over the phone if you have to do some
>minor repairs or problem solving - even if you are not mechanically minded
>- and their English puts me to shame!
>- prompt dispatch of consumable orders
>- good training course in Strasbourg
>Disadvantages
>- routine maintenance can be a bit involved; e.g. you have to dismantle
>some of it to do a vortex mix check each month and it needs a
>decontamination every 3 months
>- water quality must be good - you don't want water tanks that aren't
>cleaned out annually and you need a decent de-ioniser or you can get
>micro-organisms getting through into the XT and furring up its arteries.
>You then have to sterilise all containers (bulk and those on the XT) with
>hypochlorite bleach before making or refilling with fresh buffer
>- it doesn't like some antisera clones like the oestrogen receptor clone
>1D5; then again, some clones that didn't work too well manually or on
>other systems work well on the XT - just be aware when you are optimising
>if it doesn't work very well - try another clone
>- it may have a rather quiet alarm. Nothing could be done about it so, as
>our XT is in another room, we use a baby monitor Hope this helps
>
>Jacqui Malam
>Lancaster Infirmary
>UK
>
>
>
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>------------------------------
>
>Message: 6
>Date: Tue, 30 May 2006 07:32:07 -0400
>From: "gayle brosnanwatters" <gayle.brosnanwatters <@t> sru.edu>
>Subject: [Histonet] question
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> <8172F14C39FCAF4E99E266F6756A3683076F9653 <@t> msfexch01.srunet.sruad.edu>
>Content-Type: text/plain; charset="us-ascii"
>
>Dear Listers,
>
> I don't know if this is an appropriate question, so please
> forgive me if it is not. I have decided to retire from academia at the
> end of the school year. I am a psychology professor, but I have been
> doing research in a small way, and you folks have been an enormous help
> several times. About 6 years ago I ended up owning a brand new Leica
> sliding microtome - I believe the model is the SM2000R. (It was a fluke
>- a retiring colleague shipped his old freezing microtome to me, the
>shipper dropped it, it was insured for full value, so I got this for
>"free." I have the receipt). I know that it is identical to the one they
>sell today for about 10K, which is what I paid for it. I did not buy the
>extra freezing part, but used it with an attachment that let me use dry
>ice for frozen sectioning. Anyway, I would like to sell it. Can anyone
>tell me where I could go to do so? It was only used about 10 times, as I
>ended up using my old ultramicrotome for all the work I've done. Someone
>told me that I should expect to get no less than 5K, and because it is in
>such good condition, and it is identical to the new ones, maybe up to
>7. Does anyone have any suggestions?
>
> Again, I hope this is an appropriate question. I'd
>appreciate any help you can give me.
>
>
>
>Gayle L. Brosnan-Watters, PhD
>
>Assistant Professor
>
>Dept of Psychology
>
>Treasurer, Faculty for Undergraduate Neuroscience
>
>226 Vincent Science Hall
>
>Slippery Rock University
>
>Slippery Rock, PA 16057
>
>gayle.brosnanwatters <@t> sru.edu
>
>724-738-2529 - office
>
>724-738-4807 - fax
>
>
>
>
>
>------------------------------
>
>Message: 7
>Date: Tue, 30 May 2006 06:36:22 -0500
>From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
>Subject: RE: [Histonet] whole villi
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID:
>
><OFD2785D5F.24C38492-ON8625717E.003F5666-8625717E.003FC99F <@t> abbott.com>
>Content-Type: text/plain; charset="US-ASCII"
>
>I process mouse GI routinely looking for Caspase 3 in the villi - We cut
>short lengths of small intestine, open it, and place it (lumen up) on
>bilbous paper which has been dipped in fixative. The segments stay on the
>paper all the way through paraffin processing. To embed, I remove the
>segments off the paper, and easily embed multiple segments on edge in one
>block. The villi remain undamaged.
>Jackie O'
>
>
>
>"Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
>Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>05/26/2006 10:36 AM
>
>To
><histonet <@t> lists.utsouthwestern.edu>
>cc
>
>Subject
>RE: [Histonet] whole villi
>
>
>
>
>
>
>Renee
>Do you need to embed and cut them as a circle?
>If not I would suggest that you slit along the length and then place the
>connective tissue (or muscle) side down on a piece of card. The pieces
>should stick to the card. Then fix, remove from card and process as
>normal. Once they are fixed they should remain flat. If you have really
>big pieces can pin these to a piece of cork and float tissue side down in
>the fixative - pin using stainless steel pins, plastic pins or hedgehog
>quills. You will always have trouble in getting sections with entire
>lengths of villi in the jejunum because of the long length of these villi
>in this region. Barry
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Till, Renee
>Sent: Friday, May 26, 2006 10:24 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] whole villi
>
>I sometimes work with rat and mouse gi tissues, and as you might expect
>one of the most time consuming things is getting the tissues embedded
>good. The colon has not been as much of a problem, but lately we are doing
>more with the jejunum and getting whole villi is enough to make me scream.
>It seems to me that one of my main problems is that sometime between the
>collection of the tissues (which is not done by me) and when I embed them,
>the tissues often curl up and it is hard to get a good straight piece to
>embed. We embed them on end? (so when cut it makes a little O). Any ideas
>on how to keep them straight during the processing? Or maybe if there is
>something else that I am not thinking of that could be a problem. Thanks.
>
>
>
>Renee' Till, HT
>
>Research Assistant
>
>Arkansas Children's Nutrition Center
>
>1212 Marshall St./N2021
>
>Little Rock, AR 72002
>
>Office (501)364-2785
>
>Fax (501)364-3161
>
>
>
>
>========================================================================
>========================
>
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>------------------------------
>
>Message: 8
>Date: Tue, 30 May 2006 06:55:17 -0500
>From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
>Subject: Re: [Histonet] Ventana Benchmark
>To: "Joe Nocito" <jnocito <@t> satx.rr.com>
>Cc: histonet <@t> lists.utsouthwestern.edu,
> histonet-owner <@t> lists.utsouthwestern.edu, "'Scott A. Ely'"
> <sae2001 <@t> med.cornell.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu
>Message-ID:
>
><OF4B6E30DA.51BC187F-ON8625717E.00411200-8625717E.004184C2 <@t> abbott.com>
>Content-Type: text/plain; charset="US-ASCII"
>
>Anybody been a patient in a hospital recently? I was - and NO I'm not on
>Medicare - I have really good insurance from my husband's plan at
>Motorola.
>I was in the hospital for 4 days - my bill was over $20K - the negotiated
>payment was about 15% of the actual billed amount. I don't understand
>how hospitals are managing to stay open only recovering 10% of the actual
>cost of services. Seems our economy is driven by the insurance
>companies.
>
>
>
>
>
>"Joe Nocito" <jnocito <@t> satx.rr.com>
>Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>05/27/2006 05:56 PM
>
>To
><pruegg <@t> ihctech.net>, "'Scott A. Ely'" <sae2001 <@t> med.cornell.edu>,
><histonet <@t> lists.utsouthwestern.edu>
>cc
>histonet-owner <@t> lists.utsouthwestern.edu
>Subject
>Re: [Histonet] Ventant Benchmark
>
>
>
>
>
>
>Patsy,
>we charge what Medicare pays. Many insurance companies pay less. This is
>what fries my cookies. These people want to make money, yet pays trough
>the
>nose for immunos. Penny wise, dollar foolish is what my father would say.
>
>Joe
>----- Original Message -----
>From: <pruegg <@t> ihctech.net>
>To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>; "'Scott A. Ely'"
><sae2001 <@t> med.cornell.edu>; <histonet <@t> lists.utsouthwestern.edu>
>Cc: <histonet-owner <@t> lists.utsouthwestern.edu>
>Sent: Saturday, May 27, 2006 10:25 AM
>Subject: RE: [Histonet] Ventant Benchmark
>
>
> >I am with you Joe.
> > I have not ever been able to understand how the labs can justify
>spending
> > so
> > much to run IHC, I guess they just pass the cost on to the patient, I
>can
> > do
> > the same thing for about 1/100 of the cost of running an instrument
> > like
>
> > the
> > Ventana using an open system like the Dakoautostainer and making up a
>lot
> > of
> > my own reagents like the buffers. I work in research and don't have a
> > money
> > cow like the patient health care system to milk. No wonder health care
> > costs are so,years ago when I worked in clinical we used to do what ever
>
> > we
> > could to try and help save the patient expense, sure doesn't seem like
> > that
> > is the case anymore. I know that understaffing, quick turn around and
> > untrained personnel is a big part of the problem here but come on, do
>you
> > really want someone who only knows how to apply a bar code to be
> > running your diagnostic IHC's? Bring on the flaming......
> > Patsy
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe
>Nocito
> > Sent: Saturday, May 27, 2006 7:29 AM
> > To: Scott A. Ely; histonet <@t> lists.utsouthwestern.edu
> > Cc: histonet-owner <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Ventant Benchmark
> >
> > flaming time
> > How come every one talks about the reliability and walk away
> > technology,
>
> > but
> >
> > no one ever talks about the price of running these machines? Shouldn't
> > cost
> > be a factor also?
> > I've been in budget meetings all week. The only thing I heard was how
> > expensive it is to run immunos. Yeah, we have two XTs, what do you
>expect?
> >
> > The opinions stated here are that of the author only and does not
>express
> > the opinions of his employer, lawyers, siblings, significant others
> > and their lawyers.
> >
> > Oh, by the way, I told the same thing to my Ventana Rep on Friday. He
> > knows,
> >
> > so y'all don't need to call my CEO. Y'all know who you are.
> >
> > Let the flaming begin.
> >
> > Joe Nocito BS, HT(ASCP)QIHC
> > Histology Manager
> > Pathology Reference Lab
> > San Antonio, TX
> > ----- Original Message -----
> > From: "Scott A. Ely" <sae2001 <@t> med.cornell.edu>
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Cc: <histonet-owner <@t> lists.utsouthwestern.edu>
> > Sent: Friday, May 26, 2006 11:58 AM
> > Subject: [Histonet] Ventant Benchmark
> >
> >
> >> We are considering buying some new equipment. I would like to know
> >> who uses the Ventana Benchmark immunostainer or other immunostainers
> >> and what you like and don't like about it.
> >>
> >> Scott Ely, MD MPH
> >> Section of Hematopathology
> >> Department of Pathology
> >> Weill Medical College of Cornell University
> >> New York Presbyterian Hospital
> >> 525 E. 68th Street
> >> New York, NY 10021
> >> PH: 212-746-2442
> >>
> >> Legal Confidentiality Notice: This e-mail message, including any
> >> attachments, is for the sole use of the original intended
> >> recipient(s) selected by Dr. Ely and may contain confidential and
> >
> >> privileged information. Any
> >> unauthorized review, use, disclosure or distribution is prohibited.
> >> If
> >> you
> >
> >> are not the recipient specified by Dr.
> >> Ely, please contact the sender by reply e-mail and destroy all copies
>of
> >> the original message.
> >>
> >> ----- Original Message -----
> >> From: histonet-request <@t> lists.utsouthwestern.edu
> >> Date: Friday, May 26, 2006 12:29 pm
> >> Subject: Histonet Digest, Vol 30, Issue 39
> >>
> >>
> >
> >
> >
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> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >
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> >>
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> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> >
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> > Histonet mailing list
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 9
>Date: Tue, 30 May 2006 09:00:39 -0400
>From: Rita Riddle <RitaR <@t> lexhealth.org>
>Subject: [Histonet] Energy Beam
>To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> <CA70329E0BA39C4A974EDC102ADCE79E05802318 <@t> mail.lmc.lexhealth.org>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Can someone give me the number to Energy Beam Sciences Inc. .
>
>Thanks
>
>Rita Riddle HT(ASCP)
>Lead Histology Tech
>Lexington Medical Center
>West Columbia,SC 29169
>803-791-2881
>
>
>_________________________________
>This email and any files transmitted with it may contain PRIVILEGED or
>CONFIDENTIAL information and may be read or used only by the intended
>recipient. If you are not the intended recipient of the email or any of
>its attachments, please be advised that you have received this email in
>error and that any use, dissemination, distribution, forwarding, printing,
>or copying of this email or any attached files is strictly prohibited. If
>you have received this email in error, please immediately purge it and all
>attachments and notify the sender by reply email or contact the sender at
>the number listed above if one is provided.
>
>
>------------------------------
>
>Message: 10
>Date: Tue, 30 May 2006 09:05:17 -0400
>From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
>Subject: RE: [Histonet] Energy Beam
>To: "Rita Riddle" <RitaR <@t> lexhealth.org>,
> <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> <1CD6831EB9B26D45B0A3EAA79F7EBD3202736C77 <@t> sjhaexc02.sjha.org>
>Content-Type: text/plain; charset="utf-8"
>
>800-992-9037 or 860-653-0411
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rita Riddle
>Sent: Tuesday, May 30, 2006 9:01 AM
>To: 'histonet <@t> lists.utsouthwestern.edu'
>Subject: [Histonet] Energy Beam
>
>
>Can someone give me the number to Energy Beam Sciences Inc. .
>
>Thanks
>
>Rita Riddle HT(ASCP)
>Lead Histology Tech
>Lexington Medical Center
>West Columbia,SC 29169
>803-791-2881
>
>
>_________________________________
>This email and any files transmitted with it may contain PRIVILEGED or
>CONFIDENTIAL information and may be read or used only by the intended
>recipient. If you are not the intended recipient of the email or any of
>its attachments, please be advised that you have received this email in
>error and that any use, dissemination, distribution, forwarding, printing,
>or copying of this email or any attached files is strictly prohibited. If
>you have received this email in error, please immediately purge it and all
>attachments and notify the sender by reply email or contact the sender at
>the number listed above if one is provided.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>Confidentiality Notice ** The information contained in this message may be
>privileged and is confidential information intended for the use of the
>addressee listed above. If you are neither the intended recipient nor the
>employee or agent responsible for delivering this message to the intended
>recipient, you are hereby notified that any disclosure, copying,
>distribution or the taking of any action in reliance on the contents of
>this information is strictly prohibited. If you have received this
>communication in error, please notify us immediately by replying to the
>message and deleting it from your computer. Thank you. Saint Joseph's
>Health System, Inc.
>
>------------------------------
>
>Message: 11
>Date: Tue, 30 May 2006 08:13:20 -0500
>From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
>Subject: RE: [Histonet] Ventant Benchmark
>To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "Joe Nocito"
> <jnocito <@t> satx.rr.com>, "Malcolm McCallum"
> <Malcolm.McCallum <@t> tamut.edu>, "Scott A. Ely"
> <sae2001 <@t> med.cornell.edu>, <histonet <@t> lists.utsouthwestern.edu>
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Message-ID:
> <D6B654003615874B873E15BA680E2D221089A080 <@t> uwhis-xchng1.hosp.wisc.edu>
>Content-Type: text/plain; charset="us-ascii"
>
>We also titer a number of our antibodies, especially those infrequently
>ordered. These we don't put in Ventana dispensers but rather manually
>apply them as all the VMS instruments allow one to do.
>
>Linda Sebree, HT(ASCP)
>University of Wisconsin Hospital & Clinics
>IHC/ISH Laboratory
>A4/204-3224
>600 Highland Ave.
>Madison, WI 53792
>(608)265-6596
>FAX: (608)262-7174
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
>Sent: Sunday, May 28, 2006 12:35 PM
>To: Joe Nocito; Malcolm McCallum; Scott A. Ely;
>histonet <@t> lists.utsouthwestern.edu
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Ventant Benchmark
>
>
>That's what I was saying too. Even with all our cost cutting attempts, do
>enough immunos to support 3 reagent-leased DAKO instruments. They're walk
>away too = after you get them loaded anyway! - and the reagents are not
>nearly as expensive. We titer all the antibodies that are available
>- a savings over the prepared Abs. The company has been a great partner
>for our laboratory. j
>
>Joyce Weems
>Pathology Manager
>Saint Joseph's Hospital of Atlanta
>404-851-7376
>404-851-7831 - Fax
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Joe Nocito
>Sent: Sat 5/27/2006 6:58 PM
>To: Malcolm McCallum; Scott A. Ely; histonet <@t> lists.utsouthwestern.edu
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Ventant Benchmark
>
>I'm not saying do it manually. I'm saying that there are less expensive ways
>to perform immunos than using Ventana's high cost reagents.
>
>Joe
>----- Original Message -----
>From: "Malcolm McCallum" <Malcolm.McCallum <@t> tamut.edu>
>To: "Joe Nocito" <jnocito <@t> satx.rr.com>; "Scott A. Ely"
><sae2001 <@t> med.cornell.edu>; <histonet <@t> lists.utsouthwestern.edu>
>Cc: <histonet-owner <@t> lists.utsouthwestern.edu>
>Sent: Saturday, May 27, 2006 10:39 AM
>Subject: RE: [Histonet] Ventant Benchmark
>
>
>Undoubtedly the cost and maintenance of automation is cheaper than the cost
>of additional employees salaries and benefits accompanied by associated
>duplication of manual systems. Additionally, equipment is depreciable,
>whereas human resources are a cost.
>
>VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org
><http://www.herpconbio.org>
>
>Malcolm L. McCallum
>Assistant Professor
>Department of Biological Sciences
>Texas A&M University Texarkana
>2600 Robison Rd.
>Texarkana, TX 75501
>O: 1-903-223-3134
>H: 1-903-791-3843
>Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
>
>
>________________________________
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Joe Nocito
>Sent: Sat 5/27/2006 8:29 AM
>To: Scott A. Ely; histonet <@t> lists.utsouthwestern.edu
>Cc: histonet-owner <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Ventant Benchmark
>
>
>
>flaming time
>How come every one talks about the reliability and walk away technology,
>but no one ever talks about the price of running these machines? Shouldn't
>cost be a factor also? I've been in budget meetings all week. The only
>thing I heard was how expensive it is to run immunos. Yeah, we have two
>XTs, what do you expect?
>
>The opinions stated here are that of the author only and does not express
>the opinions of his employer, lawyers, siblings, significant others and
>their lawyers.
>
>Oh, by the way, I told the same thing to my Ventana Rep on Friday. He
>knows, so y'all don't need to call my CEO. Y'all know who you are.
>
>Let the flaming begin.
>
>Joe Nocito BS, HT(ASCP)QIHC
>Histology Manager
>Pathology Reference Lab
>San Antonio, TX
>----- Original Message -----
>From: "Scott A. Ely" <sae2001 <@t> med.cornell.edu>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Cc: <histonet-owner <@t> lists.utsouthwestern.edu>
>Sent: Friday, May 26, 2006 11:58 AM
>Subject: [Histonet] Ventant Benchmark
>
>
> > We are considering buying some new equipment. I would like to know
>who
> > uses the Ventana Benchmark
> > immunostainer or other immunostainers and what you like and don't like
> > about it.
> >
> > Scott Ely, MD MPH
> > Section of Hematopathology
> > Department of Pathology
> > Weill Medical College of Cornell University
> > New York Presbyterian Hospital
> > 525 E. 68th Street
> > New York, NY 10021
> > PH: 212-746-2442
> >
> > Legal Confidentiality Notice: This e-mail message, including any
> > attachments, is for the sole use of the original intended recipient(s)
> > selected by Dr. Ely and may contain confidential
>and
> > privileged information. Any
> > unauthorized review, use, disclosure or distribution is prohibited. If
>you
> > are not the recipient specified by Dr.
> > Ely, please contact the sender by reply e-mail and destroy all copies
>of
> > the original message.
> >
> > ----- Original Message -----
> > From: histonet-request <@t> lists.utsouthwestern.edu
> > Date: Friday, May 26, 2006 12:29 pm
> > Subject: Histonet Digest, Vol 30, Issue 39
> >
> >
>
>
>------------------------------------------------------------------------
>--------
>
>
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>------------------------------------------------------------------------
>--------
>
>
> >
>
>
>------------------------------------------------------------------------
>--------
>
>
>No virus found in this incoming message.
>Checked by AVG Free Edition.
>Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>--
>No virus found in this incoming message.
>Checked by AVG Free Edition.
>Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>Confidentiality Notice ** The information contained in this message may be
>privileged and is confidential information intended for the use of the
>addressee listed above. If you are neither the intended recipient nor the
>employee or agent responsible for delivering this message to the intended
>recipient, you are hereby notified that any disclosure, copying,
>distribution or the taking of any action in reliance on the contents of
>this information is strictly prohibited. If you have received this
>communication in error, please notify us immediately by replying to the
>message and deleting it from your computer. Thank you. Saint Joseph's
>Health System, Inc.
>
>
>
>------------------------------
>
>Message: 12
>Date: Tue, 30 May 2006 10:37:48 -0400
>From: "Rogers, Rhonda" <rhrogers <@t> iupui.edu>
>Subject: [Histonet] Cre, eGFP, and B-Gal Antibodies
>To: <histonet <@t> pathology.swmed.edu>
>Message-ID:
>
><6CB660A4F5C2D441961387F9CF60EBCA0C74A9 <@t> iu-mssg-mbx01.exchange.iu.edu>
>Content-Type: text/plain; charset="utf-8"
>
>Good morning,
>
>I will soon try Cre (from Novagen), eGFP (from Molecular Probes), and
>B-Gal (also from Molecular Probes) rabbit antibodies on formalin-fixed
>paraffin-embedded sections and/or frozen sections of mice embryos. Is
>anyone already using these antibodies for IHC staining and, if so, would
>you be willing to share your protocols?
>
>Thanks,
>
>Rhonda
>
>------------------------------
>
>Message: 13
>Date: Tue, 30 May 2006 10:17:28 -0500
>From: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
>Subject: [Histonet] slow draining paraffin dispenser
>To: <histonet <@t> pathology.swmed.edu>
>Message-ID:
>
><A4AA05CE92DACC43886D133DB94A926E211CAE8B <@t> medicine-exch1.medicine.uiowa.edu>
>
>Content-Type: text/plain; charset="us-ascii"
>
>Hi,
>
>I have Tissue Tek paraffin dispersing console Model 4586 (older model). It
>has worked well for many years, but lately it appears there is a partial
>clog, as it is dispensing much slower. Are there any (safe) protocols for
>clearing out these tubes?
>
>
>
>
>------------------------------
>
>Message: 14
>Date: Tue, 30 May 2006 11:25:44 -0400
>From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
>Subject: Re: [Histonet]Hospital costs/new topic
>To: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
>Cc: Joe Nocito <jnocito <@t> satx.rr.com>,
> histonet <@t> lists.utsouthwestern.edu,
> histonet-owner <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu, "'Scott A. Ely'"
> <sae2001 <@t> med.cornell.edu>
>Message-ID: <447C63F8.4030903 <@t> umdnj.edu>
>Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
>Hospitals don't manage "to stay open only recovering 10% of the actual
>cost of services." That would be impossible.
>They overbill to cover the costs of indigent care, improvements, etc.
>The only people who pay full price are the uninsured who have no one to
>negotiate for them.
>
>Geoff
>
>
>Jackie M O'Connor wrote:
>
> >Anybody been a patient in a hospital recently? I was - and NO I'm not
> >on
> >Medicare - I have really good insurance from my husband's plan at
> >Motorola.
> >I was in the hospital for 4 days - my bill was over $20K - the negotiated
> >payment was about 15% of the actual billed amount. I don't understand
> >how hospitals are managing to stay open only recovering 10% of the actual
> >cost of services. Seems our economy is driven by the insurance
> >companies.
> >
> >
> >
> >
> >
> >
> >
>
>
>--
>--
>**********************************************
>Geoff McAuliffe, Ph.D.
>Neuroscience and Cell Biology
>Robert Wood Johnson Medical School
>675 Hoes Lane, Piscataway, NJ 08854
>voice: (732)-235-4583; fax: -4029
>mcauliff <@t> umdnj.edu
>**********************************************
>
>
>
>
>
>------------------------------
>
>Message: 15
>Date: Tue, 30 May 2006 11:42:32 -0400
>From: "Favara, Cynthia \(NIH/NIAID\) [E]" <cfavara <@t> niaid.nih.gov>
>Subject: RE: [Histonet] slow draining paraffin dispenser
>To: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>,
> <histonet <@t> pathology.swmed.edu>
>Message-ID:
> <A985B7D45D355D4EB363288E71739437017329FF <@t> NIHCESMLBX5.nih.gov>
>Content-Type: text/plain; charset="us-ascii"
>
>Hot hair dryer might help!
>
>c
>
>Cynthia Favara
>NIAID/NIH/RML/LPVD
>903 South 4th Street
>Hamilton, MT 59840
>406-363-9317
>
>Disclaimer:
>The information in this e-mail and any of its attachments is confidential
>and may contain sensitive information. It should not be used by anyone who
>is not the original intended recipient. If you have received this e-mail
>in error please inform the sender and delete it from your mailbox or any
>other storage devices. National Institute of Allergy and Infectious
>Diseases shall not accept liability for any statements made that are
>sender's own and not expressly made on behalf of the NIAID by one of its
>representatives
>
>-----Original Message-----
>From: Walters, Katherine S [mailto:katherine-walters <@t> uiowa.edu]
>Sent: Tuesday, May 30, 2006 8:17 AM
>To: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] slow draining paraffin dispenser
>
>Hi,
>
>I have Tissue Tek paraffin dispersing console Model 4586 (older model). It
>has worked well for many years, but lately it appears there is a partial
>clog, as it is dispensing much slower. Are there any (safe) protocols for
>clearing out these tubes?
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 16
>Date: Tue, 30 May 2006 11:56:33 -0400
>From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
>Subject: RE: [Histonet] slow draining paraffin dispenser
>To: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>,
> histonet <@t> pathology.swmed.edu
>Message-ID:
>
><DEF6C218C16476458957B77B467285CD0712F6 <@t> fhosxchmb004.ADVENTISTCORP.NET>
>
>Content-Type: text/plain; charset=iso-8859-1
>
>Try cleaning out the filter disk near the bottom, inside the tank.
>
>________________________________
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Walters,
>Katherine S
>Sent: Tue 5/30/2006 11:17 AM
>To: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] slow draining paraffin dispenser
>
>
>
>Hi,
>
>I have Tissue Tek paraffin dispersing console Model 4586 (older model).
>It has worked well for many years, but lately it appears there is a
>partial clog, as it is dispensing much slower. Are there any (safe)
>protocols for clearing out these tubes?
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 17
>Date: Tue, 30 May 2006 11:04:17 -0500
>From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
>Subject: [Histonet] floaters
>To: Histonet <@t> lists.utsouthwestern.edu
>Message-ID:
> <0E394B648E5284478A6CCB78E5AFDA270171FDDB <@t> hpes1.HealthPartners.int>
>Content-Type: text/plain; charset="US-ASCII"
>
>How does everyone handle their embedding techniques in regard to
>contaminating one tissue with another? We are doing here what I have done
>at the other lab I worked at and lately the pathologist is seeing a few
>"floaters" on a slide where they shouldn't be!! Just thought I would like
>to throw this out for discussion and hopefully come away with a technique
>better than ours!! Thanks, as always!!!
>________________________________________
>This e-mail and any files transmitted with it are confidential and are
>intended solely for the use of the individual or entity to whom they are
>addressed. If you are not the intended recipient or the individual
>responsible for delivering the e-mail to the intended recipient, please be
>advised that you have received this e-mail in error and that any use,
>dissemination, forwarding, printing, or copying of this e-mail is strictly
>prohibited.
>
>If you have received this e-mail in error, please immediately notify the
>HealthPartners Support Center by telephone at (952) 967-6600. You will be
>reimbursed for reasonable costs incurred in notifying us.
>
>
>------------------------------
>
>Message: 18
>Date: Thu, 25 May 2006 18:15:53 +0200
>From: "black" <blackmail <@t> intermail.co.za>
>Subject: [Histonet] Stent paper and Histonet connection
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <000701c680a3$a0c41f30$6725d0c4 <@t> yourdbba124d3a>
>Content-Type: text/plain; charset="iso-8859-1"
>
>To all Histonetters
>
>I just thought I should mention. that the stent paper would never have
>come about, if it had not been for the connections made through Histonet.
>This is such a valuable tool for sharing of knowledge and technical
>skills, and for net working with colleagues world wide, using similar
>technology. For this paper to have become a reality, the connection was
>made via histonet, which resulted in travel to Ottawa from South Africa
>and visa versa, as well as a poster in Savannah, Georgia.
>
>There are some extremely knowledgable people who regularly contribute to
>this network, to mention a few...Gayle Kallis, Chris V D Loos, John
>Kiernan etc.etc...and many more. WWe are very fortunate to have this
>technology at our disposal. Thanks to Histonet for keeping us all up to date!!
>
>Keep it up
>Melanie Black.
>
>
>------------------------------
>
>Message: 19
>Date: Fri, 26 May 2006 16:05:37 -0400
>From: "Osborn, Barbara" <bosborn <@t> health.usf.edu>
>Subject: [Histonet] Re: methyl green counterstain
>To: <detmar <@t> mshri.on.ca>,"Histonet"
> <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>
><841D767DCDE87C49A02DA6DFC0BABABFA71F21 <@t> COMEXCHANGE.hscnet.hsc.usf.edu>
>
>Content-Type: text/plain; charset="US-ASCII"
>
>I believe it's the antigen retrieval that affects the methyl green
>counterstain. We purchase our methyl green from Vector Labs and although
>the staining is lighter than sections with no antigen retrieval, it does
>not fade out during dehydration. We counterstain for 10 min at 60oC with
>decent results.
>
>
>
>Barbara Osborn
>
>University of South Florida
>
>
>
>Message: 6
>
>Date: Thu, 25 May 2006 17:50:03 -0400
>
>From: "Jacqui Detmar" <detmar <@t> mshri.on.ca>
>
>Subject: [Histonet] methyl green counterstain
>
>To: <histonet <@t> lists.utsouthwestern.edu>
>
>Message-ID:
>
> <A249C197854D3442BDAE4C6BA4D5553C3F9E8D <@t> ex1.ad.mshri.on.ca>
>
>Content-Type: text/plain; charset="iso-8859-1"
>
>
>
>Hi all. I am doing some immunohistochemistry and TUNEL staining on mouse
>placental tissue. I have noticed that my methyl green counterstain on
>placentae assayed for TUNEL looks normal, but when I apply the same steps
>to tissue from the same block, but exposed to immunohistochemistry, the
>methyl green counterstain is very weak, bleaches out quickly during
>dehydration and generally looks pretty crappy. The methyl green recipe I
>am using is as follows: 0.5% methyl
>green in 0.1M sodium acetate buffer, pH 4.2. I would like to
>emphasize that no matter how quickly I dehydrate with the IHC sections,
>the colour is still very dim.
>
>
>
>The reason I am using methyl green is b/c I am doing IHC for nuclear
>proteins (Ki67, etc.) and don't want to use hematoxylin, since it's a
>little dark. Any suggestions?
>
>
>
>Thanks,
>
>
>
>Jacqui Detmar
>
>
>
>
>
>------------------------------
>
>Message: 20
>Date: Tue, 30 May 2006 12:33:40 -0400
>From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
>Subject: RE: [Histonet] Ventana Benchmark closed systems vs Open
> system st ainers - a diatribe
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID:
>
><0556BE8AC5551E4E8AF6BB9E42509BA20328D95C <@t> usca0082k08.labvision.apogent.com>
>
>Content-Type: text/plain
>
>Wow Brian, Spring must have hit the Great White North! The sap is starting
>to run!
>
>Brian wrote (among other things):
>
>"As technologists we all want to see the perfect machine that allows us to
>load individual slides whenever we want, process them completely, error
>free and automatically right down to the coverslip, and do it for next to
>nothing. "
>
>Brian, you forgot the imaging system! The "Automated IHC System" also has
>to deliver the coverslipped slide to an imaging system that scans the
>entire tissue section to produce a Virtual Slide. (preferably at 40X and
>10 focus levels, and then saves it (all 800 GB) to a server so anyone, anywhere
>(India?) can do the pathology consult (don't laugh, this is now happening
>in radiology)).
>
>We've actually heard this from customers. Lets, just say, yes, technically
>it can be done. But, few, if any, labs would have the half-million (US),
>or more, to buy the instrument. And you say you want it on reagent
>rental? You'd have to do thousands of slides a day to qualify. Maybe all
>the labs will eventually be consolidated into a few big labs and it will
>be possible. Not any time soon, though.
>
>Brian also wrote:
>"What I hope will happen, is that two or more companies will embrace the
>ongoing revenue stream model currently used by Ventana, and then we should
>see a rapid evolution towards truly automated IHC stainers. Competition is
>always brings out the best in industry. Look at how clinical chemistry
>analyzers now are able to process serum samples, with little need for the
>technologist to fuss with the process. That should be the goal of the IHC
>stainer manufacturer. "
>
>The dream of a totally automated IHC system for fixed tissue sections is
>not at all as simple as a chemistry or blood analysing system. In the
>clinical lab they are dealing with FRESH blood or fluid samples, all
>taken in standardized ways. The samples are nearly identical in every
>case. So a company is much more likely to be able to create instruments
>and reagents that work well over a broad range of labs.
>
>The big wrench in the IHC system is that no two labs have the same tissue
>procurement, fixation and processing system. Dr. Clive Taylor, the guru of
>standardization, has almost given up his crusade in convincing all labs to
>follow standardized procedures. For all their talk of wanting "the best"
>for their patients, no two pathologists can agree on what is "the
>best." Even the advent of "antigen retrieval" which was to overcome the
>fixation/processing variation problem, has itself become a complicated
>variable (with a slew of attendant instruments).
>
>So, considering infinite variation in tissue fixation and processing, AR
>and staining, it is nearly impossible to simply give a batch of reagents
>and an instrument to any given lab and have them all work perfectly from
>day one. In fact, every single antibody and instrument company in the
>business employs a cadre of Quality Control technologists who labor to be
>sure the antibodies sold are optimized and indeed "working," and they
>also employ a legion of "technical reps" who bravely go into unfamiliar
>labs and atttempt to "optimize" all the reagents, antibodies and protocols
>so that the system "works" in that lab. Believe me, there are very few
>labs that can do this successfully without some help.
>
>Besides that, do histotechs really want to be like Med Techs who just sit
>around looking at printouts all day long? Maybe not. We just like fiddling
>with things.
>
>
>Tim Morken
>Lab Vision - Neomarkers
>www.labvision.com
>
>Free webhosting for US State Histotechnology Societies:
>http://www.labvisioncorp.com/demowebsite/index.cfm
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Brian Chelack
>Sent: Sunday, May 28, 2006 1:55 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers
>- a diatribe
>
>Etc.
>
>Brian Chelack
>Prairie Diagnostic Services
>2604-52 Campus Drive
>Saskatoon SK
>S7N 5B4
>306-966-7241
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 21
>Date: Tue, 30 May 2006 09:43:25 -0700 (PDT)
>From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>Subject: Re: [Histonet] floaters
>To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
> Histonet <@t> lists.utsouthwestern.edu
>Message-ID: <20060530164325.8085.qmail <@t> web61219.mail.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Dorothy:
> First try to find the source of the floater.
> Compare the stained slide containing the floater with the block
> surface. If the tissue seen as floater is not in the block, it was picked
> up in the water bath. The solution is to clean the surface of the water
> with a tissue after each block is cut.
>
> If you can identify in the block the source of the floater it means
> that contamination occurred either during cassetting or during embedding.
> Make sure that the cassetting is done in a way that prevents carry-on
> of small pieces of tissue. The embedding center (the heating wells for
> the forceps) have to be empied with a pipette once embedding is finished.
> After that use a Q-tip to completely dry-clean the wells. Forceps should
> be cleaned after each block is embedded.
> Care is the solution and attention to detail is the only solution.
> Hope this will help you.
> René J.
>
>"Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
> How does everyone handle their embedding techniques in regard to
> contaminating one tissue with another? We are doing here what I have done
> at the other lab I worked at and lately the pathologist is seeing a few
> "floaters" on a slide where they shouldn't be!! Just thought I would like
> to throw this out for discussion and hopefully come away with a technique
> better than ours!! Thanks, as always!!!
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>End of Histonet Digest, Vol 30, Issue 44
>****************************************
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Mr.Laurie Reilly
School of Veterinary and Biomedical Sciences
James Cook University
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Phone 07 4781 4468
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