[Histonet] tissue processing

[Patsy Ruegg]

Is there anything bad that can happen to rat brains that have been snap
frozen in OCT if we thaw-fix them in zinc formalin and process into
paraffin?  We froze these because we were not sure the IHC would work on
ffpe tissue but have since gotten all the markers we are interested in to
work in ffpe tissue.  We would prefer to have the frozen brains in paraffin
but do not want to risk any sort of damage to antigen sites especially
and/or morphology preservation.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.