[Histonet] Feed back on bone fixation

[Reuel Cornelia]

We are fixing our bone with 70% alcohol for our research project, some
will be process for MMA(plastic) and the rest for paraffin embedding. We
will be transferring the bone tissue to 10% formalin for 24 hrs and
decalcify with EDTA for HE, Enzyme and Immunocytochemistry studies for
our paraffin embedded bone. We normally fixed the  tissue in 10%
formalin and transfer the rest of the tissue to 70% alcohol for storage.
But we never have done 70% alcohol to 10% formalin in any of our
paraffin embedded tissue. Any comments, theoretical explanation on the
effects of fixation will be helpful. 

******************************************************************************************************************* 
Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the 
treatment of orthopedic conditions and learning disorders, like dyslexia.  This email transmission 
and/or its attachments may contain confidential health information,
intended only for the use of the individual or entity named above. 

The authorized recipient of this information is prohibited from disclosing it to any other party unless 
required to do so by law and is required to delete/destroy the information after its stated need has
been fulfilled.  If you are not the intended recipient, any disclosure, copying, distribution or action 
taken in reliance on the contents of this email transmission is prohibited.  If you have received this
information in error, please notify the sender immediately and delete this information. 

We appreciate your efforts to protect the children's confidential information. 
*******************************************************************************************************************