[Histonet] Sealing Paraffin Blocks

[Jackie M O'Connor]

Sealing also deters rodents and roaches from eating the blocks - I've seen 
this in the warmer parts of the US - I've worked at some pretty old 
places.



"Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/24/2006 10:32 AM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] Sealing Paraffin Blocks






I am a strong believer in sealing blocks.
While paraffin does penetrate into cells etc. it is still possible for
water (and organisms of various types) to penetrate into the block.
After all we use water to soak into some blocks to enhance cutting.
I have seen stored, unsealed blocks, with massive penetration of fungi
throughout the entire tissue. Also if unsealed blocks are stored in a
dry atmosphere they will tend to shrink. 
If you are going to store blocks for any length of time I would seal
them not just dipping  them into molten paraffin but melting the surface
layer.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carole
Fields
Sent: Wednesday, May 24, 2006 9:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Sealing Paraffin Blocks

They say if you seal the blocks they are good much longer for special
stains, etc.  We have a ring behind the block holder (from
Richard-Allan)
that keeps everyone's microtome lined up the same.  You can also by the
Histo collimator (also from RA and I think Newcomer) to line up your
blocks
so you will not cut the tissue away on recuts.  It is a little more
costly.

Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
2720 Sunset Blvd.
W. Columbia, SC 2916

-----Original Message-----
From: Horn, Hazel V [mailto:HornHV <@t> archildrens.org]
Sent: Wednesday, May 24, 2006 9:36 AM
To: kerry.l.crabb <@t> gsk.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Sealing Paraffin Blocks


We used to do this years ago.  We no longer seal blocks.   In fact, I
believe you cut away more on a sealed block if the block requires
recutting.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
kerry.l.crabb <@t> gsk.com
Sent: Tuesday, May 23, 2006 5:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sealing Paraffin Blocks

What is the current opinion and practice on sealing paraffin blocks
prior 
to filing/archiving them?  What is the pro and con in your opinion?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------------------------------------------------
----
--
The information contained in this message may be privileged and
confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering
this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please
notify
us immediately by replying to the message and deleting it from your
computer. Thank you. 
========================================================================
====
==


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________ 
This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its
attachments, please be advised that you have received this email in
error
and that any use, dissemination, distribution, forwarding, printing, or
copying of this email or any attached files is strictly prohibited. If
you
have received this email in error, please immediately purge it and all
attachments and notify the sender by reply email or contact the sender
at
the number listed above if one is provided. 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet