[Histonet] DNA/RNA Extraction Kits for paraffinised tissues

Julie Clark JCLARK <@t> kumc.edu
Tue May 23 09:58:03 CDT 2006


Shirley,
Qiagen has kits for FFPE tissue that I have used.  We have had good
results with them.  I would recommend an overnight digestion step rather
than the 2-3 hours they recommend.  I have also used kits from Arcturus
(www.arctur.com) with LCM samples with good results (see Brian Petroff's
success story for our work).

Julie Clark
Supervisor, Molecular Pathology Laboratory
Department of Pathology
Room 2000 Hixon Mailstop 3045
Univ. of Kansas Med. Center
Kansas City, KS 66160
Office: 913-588-7254
Lab: 913-588-1793
Page: 913-917-6052
FAX: 913-588-7073

>>> Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg> 05/22/06 9:31 PM >>>
Dear All

Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, 
paraffinised tissues? I will greatly appreciate if you can share some
of 
your feedbacks. Were your yields good?

Thanks in advance!

Shirley Phua
Histopathology Laboratory
Centre for Forensic Medicine
Health Sciences Authority
DID : 62130654
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