[Histonet] RE: plant anatomy

Mon May 22 16:08:53 CDT 2006

 I use Technovit 7100 right now to section fir and pine embryos. I will
be starting fluorescence, but will embed in paraffin instead of plastic.
What is your question regarding Technovit or fluorescence? Not sure if I
can help, but I can try.

~Stephanie Brusig

Weyerhaeuser Company
Propagation of High Value Trees
32901 Weyerhaeuser Way S
WTC-1B10
Federal Way, WA 98001
253.924.6518

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Subject: Histonet Digest, Vol 30, Issue 29

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Today's Topics:

   1. Plant anatomy (Olivier Leroux)
   2. Re: Fixatives (Katri Tuomala)
   3. RE: Fixatives (Barry Madigan)

----------------------------------------------------------------------

Message: 1
Date: Sat, 20 May 2006 19:52:08 +0200
From: Olivier Leroux <Olivier.Leroux <@t> UGent.be>
Subject: [Histonet] Plant anatomy
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1148147528.446f5748c2a8c <@t> mail.ugent.be>
Content-Type: text/plain; charset=ISO-8859-1

Hello,

Is there anybody with experience on fluorescent staining?
I also wish to communicate with people who work with Technovit 7100
(Plant anatomy). I'm also searching people who would like to help me
with the localization of pectins in plant cell walls (TEM, eventually
with
immunohistochemistry)

Best regards,

Olivier

--
Olivier Leroux
http://www.pteridology.ugent.be
Olivier.Leroux <@t> UGent.be

------------------------------

Message: 2
Date: Sat, 20 May 2006 19:45:06 -0400
From: "Katri Tuomala" <katri <@t> cogeco.ca>
Subject: Re: [Histonet] Fixatives
To: "Patti Loykasek" <ploykasek <@t> phenopath.com>,	"histonet"
	<histonet <@t> pathology.swmed.edu>
Message-ID: <001801c67c67$6c8af6c0$6a9a9618 <@t> Katri>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

I totally agree with Patti. Knowing what the tissue has been fixed in
and sometimes how long would be a big help in doing special stains and
immunos. 
It should be standard information given to reference labs. I don't think
this issue belongs to Friday rants, it is a valid point!

Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Patti Loykasek" <ploykasek <@t> phenopath.com>
To: "histonet" <histonet <@t> pathology.swmed.edu>
Sent: Friday, May 19, 2006 4:21 PM
Subject: [Histonet] Fixatives

> After reading John Kiernan's eloquent dissertation on knowing your 
> fixative,
> I thought I'd venture on a similar topic. We are a reference lab and 
> receive
> specimens from all over the USA. One of my "pet peeves" is that it is
rare
> to see in the report exactly what type of fixative the specimen was 
> received
> in or subsequently processed in. I know we have no standard form of
> reporting, but it just seems like best practice to me to include this
> information on the report. One of my favorites is "...received in
> fixative..." - not very helpful. I could go on & on.
> At any rate, this is just a bit of a Friday rant. I was wondering how 
> others
> felt as perhaps I am too far in left field (or maybe just the 
> 'left"coast).
> Thanks all.
>
> Patti Loykasek
> PhenoPath Laboratories
>
>
>
------------------------------------------------------------------------
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> This e-mail message, including any attachments, is for the sole use of
> the intended recipients and may contain privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited. If
> you are not the intended recipient, please contact the sender by
e-mail
> and destroy all copies of the original message, or you may call
PhenoPath
> Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 3
Date: Sun, 21 May 2006 21:10:34 +1000
From: "Barry Madigan" <barry_m <@t> ozemail.com.au>
Subject: RE: [Histonet] Fixatives
To: "'Katri Tuomala'" <katri <@t> cogeco.ca>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c67cc7$2f08f490$0201010a <@t> WORKSTATION1>
Content-Type: text/plain;	charset="us-ascii"

Yes it would be a great help to know how long tissue has been fixed for.
Tissue that has been fixed for short times does have a tendency of being
damaged by the usual heat or enzyme antigen retrieval protocols,
particularly the nuclei detail with the heat.
I find this loss of nuclei detail a major problem when dealing with
lymphomas.
Unfortunately there is no standardisation of fixation not even in our
own
laboratory let alone the number of laboratories that refer paraffin
blocks
or slides for Immunohistochemistry.
It is a problem that is difficult to control since Immunohistochemistry
is
usually the last area to deal with cases before the reports are
finalised.
Also there is the added problem that your control material has not
received
the same conditions as the test.
Thank God for internal controls.

Well that was my rant.

Barry Madigan
Immunohistochemistry
QHPS-Central
Queensland
Australia

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Katri
Tuomala
Sent: Sunday, 21 May 2006 9:45 AM
To: Patti Loykasek; histonet
Subject: Re: [Histonet] Fixatives

I totally agree with Patti. Knowing what the tissue has been fixed in
and 
sometimes how long would be a big help in doing special stains and
immunos. 
It should be standard information given to reference labs. I don't think

this issue belongs to Friday rants, it is a valid point!

Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Patti Loykasek" <ploykasek <@t> phenopath.com>
To: "histonet" <histonet <@t> pathology.swmed.edu>
Sent: Friday, May 19, 2006 4:21 PM
Subject: [Histonet] Fixatives

> After reading John Kiernan's eloquent dissertation on knowing your 
> fixative,
> I thought I'd venture on a similar topic. We are a reference lab and 
> receive
> specimens from all over the USA. One of my "pet peeves" is that it is
rare
> to see in the report exactly what type of fixative the specimen was 
> received
> in or subsequently processed in. I know we have no standard form of
> reporting, but it just seems like best practice to me to include this
> information on the report. One of my favorites is "...received in
> fixative..." - not very helpful. I could go on & on.
> At any rate, this is just a bit of a Friday rant. I was wondering how 
> others
> felt as perhaps I am too far in left field (or maybe just the 
> 'left"coast).
> Thanks all.
>
> Patti Loykasek
> PhenoPath Laboratories
>
>
>
------------------------------------------------------------------------
-
> This e-mail message, including any attachments, is for the sole use of
> the intended recipients and may contain privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited. If
> you are not the intended recipient, please contact the sender by
e-mail
> and destroy all copies of the original message, or you may call
PhenoPath
> Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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