[Histonet] disinfecting cryostat
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Mon May 22 09:35:23 CDT 2006
Bleach is a tuberculocide.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
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Sent: Friday, May 19, 2006 11:09
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 30, Issue 26
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Today's Topics:
1. rabbit anti-FITC (Elizabeth Chlipala)
2. Help workers comp issue (Michelle D. Moore)
3. Re: Help workers comp issue (Jackie M O'Connor)
4. Good antibodies for macrophage detection in rat samples
(Guillermo Palao)
5. Re: disinfecting the cryostat (Robyn Vazquez)
6. RE: Good antibodies for macrophage detection in rat samples
(Elizabeth Chlipala)
7. section thickness for IHC (Osborn, Sharon)
8. specimen size for IHC (Heather Renko)
9. Re: specimen size for IHC (Jackie M O'Connor)
10. Histotech Position in Richmond, VA (Paula Sicurello)
11. rabbit anti-GFP FITC labeled (Elizabeth Chlipala)
12. RE: immunofluorescence amplification... (Andrea T. Hooper)
13. FW: Jb-4 blocks (Allen, Rhonda)
14. Source of Re: [Histonet] IHC - Mycoplasma Ab (Gayle Callis)
15. Re: Sakura Xpress users (Lori Richey)
16. Re: rabbit anti-FITC (Gayle Callis)
17. Re: Good antibodies for macrophage detection in rat samples
(Gayle Callis)
18. RE: Sakura Xpress users (Bartlett, Jeanine (CDC/NCID/VR))
19. Re: rabbit anti-FITC (Andrea T. Hooper)
20. Her 2 question (BSylinda <@t> aol.com)
21. Re: section thickness (Katri Tuomala)
22. Please unsubscibe me from your list server. Thank you.
(Barb Nuernberger)
23. Beta-catenin (Jairam Vanamala)
24. Re: Incubation chamber (Geoffrey White)
25. RE: FW: Jb-4 blocks (Edmondson David (RBV) NHS Christie Tr)
26. unsubscribe (Johnson, Cindy, M)
27. (no subject) (ashokc <@t> ncbs.res.in)
28. Histology Position (Patterson, Pat)
----------------------------------------------------------------------
Message: 1
Date: Thu, 18 May 2006 10:59:55 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] rabbit anti-FITC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c67a9c$7e6a7270$0300a8c0 <@t> Chlipala>
Content-Type: text/plain; charset="us-ascii"
Hello everyone
I was searching the histonet archives and came across an e-mail from
Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC
labeled mouse antibodies on mouse tissue. To make a long story short,
it seems that Dako has discontiued this item, is anyone aware of a good
subsititue for this, the one I was interested in was not biotinylated.
Thanks in advance
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/>
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
------------------------------
Message: 2
Date: Thu, 18 May 2006 11:01:43 -0600
From: "Michelle D. Moore" <tmhpath <@t> amigo.net>
Subject: [Histonet] Help workers comp issue
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c67a9c$bde339f0$ef00a8c0 <@t> tmhpath1>
Content-Type: text/plain; charset="iso-8859-1"
Hello fellow colleagues,
I am at a point of frustration with workers comp, some surprise there huh? I need to be pointed in the direction of getting information about histotechs and repetitive motion disorders. I have a tear on my tendon in my right elbow with epicondylitis and bilateral carpal tunnel syndrome and of course they are saying it is not work related (BULL). I did not have this problem until working in histology and I do not play any sports or anything that would cause these problems. I would really appreciate some help with this as I am going to have to get a lawyer to even get anything done about this. I appreciate all your time and help with this. Sorry to vent but I just got off the phone with workers comp and I am angry and hurt. Thank you again for your time and help.
Michelle D. Moore HT(ASCP)
The Memorial Hospital
785 Russell St
Craig, CO 81625
tmhpath <@t> amigo.net
970-826-3292
Fax 970-826-3158
------------------------------
Message: 3
Date: Thu, 18 May 2006 12:19:47 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Help workers comp issue
To: "Michelle D. Moore" <tmhpath <@t> amigo.net>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OFB162E6C3.9C3B662E-ON86257172.005E00FD-86257172.005F3A0D <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Michelle -
Speaking as someone who has suffered from tendinitis from microtomy, who
says it's not work related? Workmans comp? What is your claim for? Missed
work, health care costs, or other damages? What does your doctor say?
You'll need supporting documentation from your ortho for any action to be
taken seriously.
It sounds as if your place of employment isn't cooperating - I slammed my
finger in a drawer at work a couple of months ago, and did some nerve
damage - I didn't go to employee health until a week later because I
thought it was stupid, but then they were all over it - even sent me to a
hand specialist. The feeling eventually did come back in my finger (I can
tell, you were ALL worried) - anyway - it's basically the doctor's call -
and he'll recommend how to prevent additional damage, which probably
includes adapting your microtomy style, among other things. Then you
probably could get a good personal injury lawyer who will do all the
research for 30% of your settlement.
Good luck.
"Michelle D. Moore" <tmhpath <@t> amigo.net>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/18/2006 12:01 PM
To
"Histonet" <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Help workers comp issue
Hello fellow colleagues,
I am at a point of frustration with workers comp, some surprise there
huh? I need to be pointed in the direction of getting information about
histotechs and repetitive motion disorders. I have a tear on my tendon in
my right elbow with epicondylitis and bilateral carpal tunnel syndrome and
of course they are saying it is not work related (BULL). I did not have
this problem until working in histology and I do not play any sports or
anything that would cause these problems. I would really appreciate some
help with this as I am going to have to get a lawyer to even get anything
done about this. I appreciate all your time and help with this. Sorry to
vent but I just got off the phone with workers comp and I am angry and
hurt. Thank you again for your time and help.
Michelle D. Moore HT(ASCP)
The Memorial Hospital
785 Russell St
Craig, CO 81625
tmhpath <@t> amigo.net
970-826-3292
Fax 970-826-3158
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 18 May 2006 20:00:41 +0200 (CEST)
From: Guillermo Palao <gpbnas <@t> yahoo.es>
Subject: [Histonet] Good antibodies for macrophage detection in rat
samples
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20060518180041.17105.qmail <@t> web26206.mail.ukl.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello all,
We are interested in detecting macrophages in FFPE samples from rat. I would really appreciate any suggestions regarding the best antigen (Is F4-80 the best?) and most useful commercial antibody to use.
Thanks in advance,
Guillermo
---------------------------------
LLama Gratis a cualquier PC del Mundo.
Llamadas a fijos y móviles desde 1 céntimo por minuto.
http://es.voice.yahoo.com
------------------------------
Message: 5
Date: Thu, 18 May 2006 11:00:46 -0700
From: "Robyn Vazquez" <VAZQUEZR <@t> ohsu.edu>
Subject: Re: [Histonet] disinfecting the cryostat
To: delventh3 <@t> aol.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <s46c53f1.095 <@t> OHSU.EDU>
Content-Type: text/plain; charset=us-ascii
We use a product that is a broad-spectrum, SaniMaster IV Google it. Shut cryo down, drench inside with diluted SaniMaster IV, let set for awhile, drain, wipe out, spray with 100%, wipe out, air dry for awhile, plug back in, wah la...tried calling number, but got a not in service announcement.
Hope this helps
Robyn
OHSU
>>> <delventh3 <@t> aol.com> 5/18/2006 8:56 AM >>>
Does anyone have a protocol for disinfecting the cryostat? Specifically a substance that is specific for AFB. If you could give a call to anyone of us at the Histology Lab we would appreciate it. Thanks in advance.
Priscilla
Histology, St. Anthony's Hospital, Rockford, Ill. 815-395-3410
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------------------------------
Message: 6
Date: Thu, 18 May 2006 12:09:17 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Good antibodies for macrophage detection in
rat samples
To: "'Guillermo Palao'" <gpbnas <@t> yahoo.es>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c67aa6$2df0e680$0300a8c0 <@t> Chlipala>
Content-Type: text/plain; charset="iso-8859-1"
F4/80 is a murine macrophage marker. I use ED-1 from serotec, it's a
mouse anti-rat macrophage marker. It works well on FFPE tissue with
proteinase K digestion, (even works on formic acid decalcifed bone).
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Guillermo Palao
Sent: Thursday, May 18, 2006 12:01 PM
To: Histonet
Subject: [Histonet] Good antibodies for macrophage detection in rat
samples
Hello all,
We are interested in detecting macrophages in FFPE samples from rat. I
would really appreciate any suggestions regarding the best antigen (Is
F4-80 the best?) and most useful commercial antibody to use.
Thanks in advance,
Guillermo
---------------------------------
LLama Gratis a cualquier PC del Mundo.
Llamadas a fijos y móviles desde 1 céntimo por minuto.
http://es.voice.yahoo.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Thu, 18 May 2006 14:17:13 -0400
From: "Osborn, Sharon" <SHARON.OSBORN <@t> SPCORP.COM>
Subject: [Histonet] section thickness for IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9A919A5D70313A4D9C56A025710874080C72B2 <@t> kenmsg40.us.schp.com>
Content-Type: text/plain; charset="us-ascii"
We do ours at 5 microns, brains, CNS are at 10-15 microns
sharon osborn
DNAX, SP BioPharma
Palo Alto, CA
Subject: [Histonet] section thickness
In your invaluable, but, no doubt differing opinions, what is the
optimal section thickness for formalin fixed paraffin processed
tissues for immunostaining, using DAB as chromogen?, there has been
some discussion here whether it should be 4,5,6, or 7microns, many
thanks.
Richard Edwards
MRC TOXICOLOGY UNIT
LEICESTER..U.K.
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Message: 8
Date: Thu, 18 May 2006 11:45:14 -0700 (PDT)
From: Heather Renko <omnivore98 <@t> yahoo.com>
Subject: [Histonet] specimen size for IHC
To: histonet <@t> pathology.swmed.edu
Message-ID: <20060518184514.12226.qmail <@t> web31305.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process. The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6.
Heather Renko
In your invaluable, but, no doubt differing opinions, what is the
optimal section thickness for formalin fixed paraffin processed
tissues for immunostaining, using DAB as chromogen?, there has been
some discussion here whether it should be 4,5,6, or 7microns, many
thanks.
Richard Edwards
---------------------------------
Ring'em or ping'em. Make PC-to-phone calls as low as 1¢/min with Yahoo! Messenger with Voice.
------------------------------
Message: 9
Date: Thu, 18 May 2006 14:34:55 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] specimen size for IHC
To: Heather Renko <omnivore98 <@t> yahoo.com>
Cc: histonet <@t> pathology.swmed.edu
Message-ID:
<OFFCF7BAFA.A9B83B1B-ON86257172.006AFA95-86257172.006B9936 <@t> abbott.com>
Content-Type: text/plain; charset="UTF-8"
CM Van der Loos is the "Dr. House of Histology". I believe whatever he
says unequivocally.
I pulled this from the Jan 06 archives.
[Histonet] RE: optimal thickness for cutting of IHC sections
From:
"C.M. van der Loos"
Dear Paul,
I totally agree what you
wrote about IHC: it's just a surface event.
Long time ago we had to prove that our (prehistoric) attempt of
quantifying an IHC technique wasn't influenced by tissue thickness
due to microtome abberations. We did cut (cryostat) sections of
different thickness and guess what: there was hardly any influence
on the staining intensity. The only thing we observed was
that thicker sections showed a higher non-specific background
staining than thinner sections. See:
CM van der Lo os, MMH Marijianowski and AE Becker:
Quantification in
immunohistochemistry. The measurement of the ratioâEUR(tm)s between
collagen types I and I II. Histochem J (1994) 26,
347-354<= /P>
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
M 2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Nether lands
Date: Tue, 3 Jan 2006 13:09:20 -0500
From:
"Monfils, Paul"
<PMonfils <@t> Lifespan.org>
Subject : RE:
[Histonet] optimal thickness for cutting of IHC sections< BR>To:
'histonet <@t> lists.utsouthwestern.edu'
I believe section thickness is less critical for IHC
because antibodies are very large molecules that don't
penetrate tissue very well, so regardless of
the thickness of the section, you are really only staining
the
exposed surface of the tissue, perhaps to a depth of 2
microns or so. We have verified this by electron
microscopy. This is quite different from standard histochemical
procedures where a thicker section results in a
more intense stain because the small dye molecules penetrate
the tissue readily and stain it all the way through.
Heather Renko <omnivore98 <@t> yahoo.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/18/2006 01:45 PM
To
histonet <@t> pathology.swmed.edu
cc
Subject
[Histonet] specimen size for IHC
The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate
penetration in the fixation process. The Immunochemical Staining
handbook(3rd Ed, published by Dako) states to cut at 4-6.
Heather Renko
In your invaluable, but, no doubt differing opinions, what is the
optimal section thickness for formalin fixed paraffin processed
tissues for immunostaining, using DAB as chromogen?, there has been
some discussion here whether it should be 4,5,6, or 7microns, many
thanks.
Richard Edwards
---------------------------------
Ring'em or ping'em. Make PC-to-phone calls as low as 1¢/min with Yahoo!
Messenger with Voice.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 18 May 2006 16:16:24 -0400
From: "Paula Sicurello" <psicurello <@t> mcvh-vcu.edu>
Subject: [Histonet] Histotech Position in Richmond, VA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF6F5541E6.19AE3056-ON85257172.006F5D69-85257172.006F5D72 <@t> MCVH-VCU.EDU>
Content-Type: text/plain; charset="iso-8859-1"
Hello Fellow Histologists,
The Medical College of Virgini histotechnologists. We have 2 hi
The posting only shows the one position as yet, but there will be 2 jobs.
Please go to [1]www.vcuhealth.org and look under the Allied Health section of the job listings. It lists the hours but I don't know how set those are.
If you're interested, you can e-mail me a copy of your resume and I'll pass it along to the powers that be. Also, please fill out the
o
Thanks to all who are interested,
Paula :-)
References
1. 3D"http://www.vcuhealth.org"=/
------------------------------
Message: 11
Date: Thu, 18 May 2006 14:23:47 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] rabbit anti-GFP FITC labeled
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c67ab8$f7d4d710$0300a8c0 <@t> Chlipala>
Content-Type: text/plain; charset="us-ascii"
Hello
Does anyone out there know of a good rabbit anti-GFP that FITC labeled.
The client I'm working with wants an affinity purified antibody if
possible.
Thanks in advance.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/>
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
------------------------------
Message: 12
Date: Thu, 18 May 2006 16:59:15 -0400
From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Subject: [Histonet] RE: immunofluorescence amplification...
To: histonet <@t> pathology.swmed.edu
Message-ID: <p06200716c0928fa1961e@[10.0.1.4]>
Content-Type: text/plain; charset=us-ascii; format=flowed
I find using a biotinylated secondary and Streptavidin labeled with
fluorophore also works well for amplication, certainly a step up from
directly labeled secondaries where amplification is desired.
Good luck!
Andrea
At 8:56 AM +0200 5/17/06, C.M. van der Loos wrote:
>
>
> Date: Mon, 15 May 2006 16:44:28 -0700 (PDT)
> From: Adam Perry <kaleid11 <@t> yahoo.com>
> Subject: [Histonet] immunofluorescence amplification...
> To: histonet <@t> pathology.swmed.edu
> What are people's impressions about different (i.e. the best) ways to
> amplify fluorescent signal from immunocytochemistry?
> I'm working with 30 um rodent brain sections. When I use a
> biotinylated secondary antibody with avidin-biotin-HRP detection with
> DAB or NiDAB I get good cellular staining with a primary antibody
> concentration of 1:1,000.
> I am now trying to perform double labeling and so have switched to
> fluorescence...and I don't seem to be getting any specific signal
> now.
> I have tried increasing the antibody concentration from 1:1,000 up to
> 1:100 and still don't see the faintest hint of staining (and the
> background just gets really bad).
> I've used the fluorescent antibody to detect other primaries (so the
> secondary is working in general and my mi! croscope
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
------------------------------
Message: 13
Date: Thu, 18 May 2006 16:45:27 -0500
From: "Allen, Rhonda" <RRA <@t> Stowers-Institute.org>
Subject: [Histonet] FW: Jb-4 blocks
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E7804FB2139 <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
> Histonetter's,
>
> The following question was posted on the microscopy list serve by a
> friend. I thought maybe some of the folks in histoland might have
> some good suggestions.
>
> Rhonda Allen
>
>
> "We are doing a rush job for a client who requires 4.0 um sections
> from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is
> having a dickens of a time getting the sections to remain flat when
> removing them from the knife. She is cutting on glass and taking
> sections from the dry edge with a fine forceps. As soon as the
> sections leave the knife, they curl and won't uncurl when placed on a
> drop of water on a slide.
>
> Not only is this a rush job in support of a grant proposal, but it
> requires serial sectioning with no missing sections, and we have,
> like,
> no real experience with this resin. Cheryl has tried various sized
> block faces and different thicknesses for the sections, but nothing is
> helping.
>
> The thumping sound you hear is a head hitting a wall----repeatedly.
> Can anyone HEEEELLLLP??"
>
>
------------------------------
Message: 14
Date: Thu, 18 May 2006 16:00:52 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Source of Re: [Histonet] IHC - Mycoplasma Ab
To: "Jan Shivers" <shive003 <@t> umn.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060518155847.01b31680 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Richard Cartun gave out a fabulous source, ViroStat out of Portland
Maine. Just go to ViroStat website and shop -
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 15
Date: Thu, 18 May 2006 15:01:10 -0700
From: Lori Richey <lrichey <@t> u.washington.edu>
Subject: Re: [Histonet] Sakura Xpress users
To: Angela Bitting <akbitting <@t> geisinger.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <446CEEA6.3090006 <@t> u.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
We use the xpress processor for most biopsy specimens like liver,
cervical, prostate, and oral path. Our pathologists have not agreed to
put the renal, skin biopsies or the breast biopsies on the express. We
also still run all of the regular surgical specimens on the VIP. These
work well on the xpress, however, they must be cut in at the recommended
size limits. Also, due to the fixation time in formalin for most of the
large specimens it works better to load them in the VIP in formalin for
a couple hours before the processor runs. There are only 2 end chambers
on the Xpress, meaning someone has to be present to unload it.
Most of the special stains and the IHC staining is comparable to routine
processing. The Trich stain on liver biopsies done on the Artisan
special stainer has been the most challenging.
Angela Bitting wrote:
>I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it?
>
>Angela Bitting, HT(ASCP)
>Technical Specialist, Histology
>Geisinger Medical Center
>100 N Academy Ave. MC 23-00
>Danville, PA 17822
>phone 570-214-9634
>fax 570-271-5916
>
>
>
>IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
>
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 16
Date: Thu, 18 May 2006 16:10:05 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] rabbit anti-FITC
To: "Elizabeth Chlipala" <liz <@t> premierlab.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060518160705.01b39f00 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
This is sad as this antibody worked so nicely. It worked beautifully with
Rat antMouse CD4-FITC. Chris van der Loos was the one who clued me in to
this antibody, as he used it cleverly for his double staining. Maybe he
has a new source for one of equal quality.
Gayle Callis
At 10:59 AM 5/18/2006, you wrote:
>Hello everyone
>
>I was searching the histonet archives and came across an e-mail from
>Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC
>labeled mouse antibodies on mouse tissue. To make a long story short,
>it seems that Dako has discontiued this item, is anyone aware of a good
>subsititue for this, the one I was interested in was not biotinylated.
>
>Thanks in advance
>
>Liz
>
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>Manager
>Premier Laboratory, LLC
>P.O. Box 18592
>Boulder, Colorado 80308
>Office: (303) 735-5001
>Fax: (303) 735-3540
>liz <@t> premierlab.com
>www.premierlab.com <http://www.premierlab.com/>
>
>Ship to Address:
>Premier Laboratory
>University of Colorado
>MCDB, Room A3B40
>Boulder, Colorado 80309
>
>
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 18 May 2006 16:15:42 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Good antibodies for macrophage detection in
rat samples
To: Guillermo Palao <gpbnas <@t> yahoo.es>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060518161117.01b49008 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed
F4-80 ( a rat antimouse monoclonal) is for mouse macrophages, not
rat. There are several well documented rat macrophage antibodies, go to
Serotec and look for these. ED1, ED2, ED3 are subsets. These mouse
antiRat monoclonals were detected with goat antimouse secondaries that
mouse IgG isotypes (IgG1, etc) biotinylated from Southern Biotechnology.
At 12:00 PM 5/18/2006, you wrote:
>Hello all,
>
> We are interested in detecting macrophages in FFPE samples from rat. I
> would really appreciate any suggestions regarding the best antigen (Is
> F4-80 the best?) and most useful commercial antibody to use.
>
> Thanks in advance,
>
> Guillermo
>
>
>---------------------------------
>
>LLama Gratis a cualquier PC del Mundo.
>Llamadas a fijos y móviles desde 1 céntimo por minuto.
>http://es.voice.yahoo.com
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 18
Date: Thu, 18 May 2006 18:29:05 -0400
From: "Bartlett, Jeanine \(CDC/NCID/VR\)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Sakura Xpress users
To: "Lori Richey" <lrichey <@t> u.washington.edu>, "Angela Bitting"
<akbitting <@t> geisinger.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CB857F6460D42E4AAEA195054A25406C0ACC324B <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="UTF-8"
Lori:
What is the conflict with the pathologists regarding the renal, skin and breast biopsies being run on the Xpress?
Thanks.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Lori Richey
Sent: Thu 5/18/2006 6:01 PM
To: Angela Bitting
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sakura Xpress users
We use the xpress processor for most biopsy specimens like liver,
cervical, prostate, and oral path. Our pathologists have not agreed to
put the renal, skin biopsies or the breast biopsies on the express. We
also still run all of the regular surgical specimens on the VIP. These
work well on the xpress, however, they must be cut in at the recommended
size limits. Also, due to the fixation time in formalin for most of the
large specimens it works better to load them in the VIP in formalin for
a couple hours before the processor runs. There are only 2 end chambers
on the Xpress, meaning someone has to be present to unload it.
Most of the special stains and the IHC staining is comparable to routine
processing. The Trich stain on liver biopsies done on the Artisan
special stainer has been the most challenging.
Angela Bitting wrote:
>I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it?
>
>Angela Bitting, HT(ASCP)
>Technical Specialist, Histology
>Geisinger Medical Center
>100 N Academy Ave. MC 23-00
>Danville, PA 17822
>phone 570-214-9634
>fax 570-271-5916
>
>
>
>IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
>
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>
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Thu, 18 May 2006 18:36:17 -0400
From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Subject: Re: [Histonet] rabbit anti-FITC
To: Histonet <histonet <@t> lists.utsouthwestern.edu>, liz <@t> premierlab.com
Message-ID: <p0620071cc092a4a382aa@[10.0.1.4]>
Content-Type: text/plain; charset=us-ascii; format=flowed
Bummer, I loved this antibody also for mouse on mouse staining.
DAKO sells a mouse anti-FITC, clone DAK-FITC4, which is excellent for
staining human tissue with FITC labeled antibodies using the Mouse
EnVision+ kit as the detection system. I am hoping this antibody was
not discontinued as well!
Also FYI, there is a sheep anti-FITC-HRP (from the TUNEL POD kit but
sold separately also) from Roche Molecular Biochemicals which is
excellent and I routintely use it for conversion of good strong
labeling FITC antibodies to HRP/DAB detection. (The primary must
obviously stain robustly since there is a lack of amplification
using this protocol).
With just a quick scan of InVitrogen/Zymed/Molecular Probes website
.... I see a rabbit anti-FITC from Zymed Catalog #71-1900, I know
nothing about its quality though, probably decent!
-- Andrea
>At 10:59 AM 5/18/2006, you wrote:
>>Hello everyone
>>
>>I was searching the histonet archives and came across an e-mail from
>>Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC
>>labeled mouse antibodies on mouse tissue. To make a long story short,
>>it seems that Dako has discontiued this item, is anyone aware of a good
>>subsititue for this, the one I was interested in was not biotinylated.
>>
>>Thanks in advance
>>
>>Liz
>>
>>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>>Manager
>>Premier Laboratory, LLC
>>P.O. Box 18592
>>Boulder, Colorado 80308
>>Office: (303) 735-5001
>>Fax: (303) 735-3540
>>liz <@t> premierlab.com
>>www.premierlab.com <http://www.premierlab.com/>
--
------------------------------
Message: 20
Date: Thu, 18 May 2006 19:27:37 EDT
From: BSylinda <@t> aol.com
Subject: [Histonet] Her 2 question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <3e5.2d9bf9d.319e5ce9 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello Histoland,
Can anyone out there can give me some info about if you are using a multi
control for Her 2. We currently send out Her 2, and we are getting a single 1
punch control equal to a 3 plus control. My question is how do you run a 3
plus control in house and without running a multi control which has 0, 1+,
and 3+.
All feedback will be greatly appreciated,
Thanks in advance
Sylinda Battle HT ASCP
------------------------------
Message: 21
Date: Thu, 18 May 2006 21:30:34 -0400
From: "Katri Tuomala" <katri <@t> cogeco.ca>
Subject: Re: [Histonet] section thickness
To: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <002b01c67ae3$d35adcb0$6a9a9618 <@t> Katri>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Richard,
I do all my immunos at 3 um, always have for the last 20 years...
Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
To: <histonet <@t> pathology.swmed.edu>
Sent: Thursday, May 18, 2006 6:43 AM
Subject: [Histonet] section thickness
In your invaluable, but, no doubt differing opinions, what is the
optimal section thickness for formalin fixed paraffin processed tissues
for immunostaining, using DAB as chromogen?, there has been some
discussion here whether it should be 4,5,6, or 7microns, many thanks.
Richard Edwards
MRC TOXICOLOGY UNIT
LEICESTER..U.K.......
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------------------------------
Message: 22
Date: Thu, 18 May 2006 21:45:24 -0400
From: "Barb Nuernberger" <BNuern <@t> coj.net>
Subject: [Histonet] Please unsubscibe me from your list server. Thank
you.
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s46ceb04.034 <@t> mail.itd.ci.jax.fl.us>
Content-Type: text/plain; charset=US-ASCII
Please unsubscibe me from your list server. Thank you.
Barb Nuernberger
------------------------------
Message: 23
Date: Thu, 18 May 2006 19:23:39 -0700 (PDT)
From: Jairam Vanamala <vanamalajl <@t> yahoo.com>
Subject: [Histonet] Beta-catenin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060519022339.56541.qmail <@t> web31806.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone has the working IHC protocol for beta-catenin, preferably for rat colon.
Jairam Vanamala
Post-doc
TAMU
College Station, TX
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------------------------------
Message: 24
Date: Fri, 19 May 2006 05:52:10 -0700 (PDT)
From: Geoffrey White <drgeoffreywhite <@t> yahoo.com>
Subject: Re: [Histonet] Incubation chamber
To: Histonet <@t> lists.utsouthwestern.edu
Cc: jatindra_prakash <@t> yahoo.com
Message-ID: <20060519125210.64906.qmail <@t> web38107.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Try out QInstruments.
There you will find a new incubation chamber for a little price.
Good Luck
Geoffrey
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------------------------------
Message: 25
Date: Fri, 19 May 2006 14:35:44 +0100
From: "Edmondson David \(RBV\) NHS Christie Tr"
<David.Edmondson <@t> christie-tr.nwest.nhs.uk>
Subject: RE: [Histonet] FW: Jb-4 blocks
To: "Allen, Rhonda" <RRA <@t> Stowers-Institute.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3C83687E8F6AE04792E361ABE2D385B8418115 <@t> cht-mail2-2k.xchristie.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Hi,
It's years ago, but I remember something along the lines of 70% alc, and
picking up the section with a brush in a droplet of the alcohol and
flicking it onto a staining pot of water. They span and the creases
came out . Then picked up onto slides and dried on a hotplate.
Does that approach sound familiar to anyone?
David
Christie Hosp
Manchester UK
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Allen,
Rhonda
Sent: 18 May 2006 22:45
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: Jb-4 blocks
> Histonetter's,
>
> The following question was posted on the microscopy list serve by a
> friend. I thought maybe some of the folks in histoland might have
> some good suggestions.
>
> Rhonda Allen
>
>
> "We are doing a rush job for a client who requires 4.0 um sections
> from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is
> having a dickens of a time getting the sections to remain flat when
> removing them from the knife. She is cutting on glass and taking
> sections from the dry edge with a fine forceps. As soon as the
> sections leave the knife, they curl and won't uncurl when placed on a
> drop of water on a slide.
>
> Not only is this a rush job in support of a grant proposal, but it
> requires serial sectioning with no missing sections, and we have,
> like,
> no real experience with this resin. Cheryl has tried various sized
> block faces and different thicknesses for the sections, but nothing is
> helping.
>
> The thumping sound you hear is a head hitting a wall----repeatedly.
> Can anyone HEEEELLLLP??"
>
>
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Message: 26
Date: Fri, 19 May 2006 08:57:32 -0500
From: "Johnson, Cindy, M" <cmjohnson <@t> cmh.edu>
Subject: [Histonet] unsubscribe
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FD31681A0E94D04F9824B252A7A2CC6420451D <@t> exchmail.CMH.Internal>
Content-Type: text/plain; charset="us-ascii"
Please unsubscribe me from your list server. Thank you.
------------------------------
Message: 27
Date: Fri, 19 May 2006 19:46:09 +0530 (IST)
From: ashokc <@t> ncbs.res.in
Subject: [Histonet] (no subject)
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <1249.59.92.206.50.1148048169.squirrel <@t> 59.92.206.50>
Content-Type: text/plain;charset=iso-8859-1
please unsubscribe from histonet
Ashok.C
Research Scholar
Prof.M.M.Panicker's lab
Molecular Neurobiology
National Centre for Biological Sciences
GKVK Campus
Bangalore-560065
Phone-+91-080-23636421 ext :2251
Fax no-91-080-23636662
------------------------------
Message: 28
Date: Fri, 19 May 2006 09:52:59 -0500
From: "Patterson, Pat" <PatPatterson <@t> mhd.com>
Subject: [Histonet] Histology Position
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <293C7C19EFF7D611AE1A0002A53F8114164AC225 <@t> omega.mhd.com>
Content-Type: text/plain; charset="us-ascii"
Methodist Dallas Medical Center - voted "One of the Best Places to Work
in Dallas" currently has a full-time 2nd shift position available for a
certified Histology Technician/Technologist. Experience in all aspects
of Histology are required. Frozen section and Immunofluoresence
experience is desired.
We also have a PRN position available for either 1st or 2nd shift hours.
Contact : Pat Patterson
214-947-3538
patpatterson <@t> mhd.com
OR Lorena Davila, HR recruiter
lorenadavila <@t> mhd.com
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