[Histonet] section thickness

[Dawson, Glen]

I do all of my immunostaining @ 3 microns.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Edwards,
R.E.
Sent: Thursday, May 18, 2006 4:44 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] section thickness


 
 
 
In your  invaluable, but,  no  doubt differing opinions, what  is  the  optimal section thickness   for formalin fixed paraffin processed tissues for  immunostaining, using  DAB  as  chromogen?, there has been  some  discussion here  whether  it  should be 4,5,6, or  7microns, many thanks.
 
                                                                                               Richard  Edwards
 
                                                                                                MRC TOXICOLOGY UNIT
                                                                                                         LEICESTER..U.K.......
 
 
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