[Histonet] 34BE12

[Roxanne Soto]

   Thank you Glen,

   This  is  what  I have been doing...and I too have found that there is
   always  some  staining,  there  is always some small portion of normal
   tissue.   It  is  highly  unlikely that the entire core is completlely
   malignant.   The  only  diffence  is  that  we  do  not  do a negative
   control......I  know  what CAP says.  But we are not CAP.  The doctors
   feel  that  since it is staining for normal tissue, a negative control
   is  not  necessary. Even  on  our  other  antibodies,  we  do not do a
   negative  control per block--we only do a negative per patient......if
   out  patient  has 12 blocks and each has an antibody ordered, we still
   only do 1 negative.

   Any suggestions?

   Roxanne
       ______________________________________________________________

     From:  "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
     CC:  histonet-bounces <@t> lists.utsouthwestern.edu,
     HISTONET <@t> PATHOLOGY.SWMED.EDU
     Subject:  RE: [Histonet] 34BE12
     Date:  Tue, 16 May 2006 12:43:13 -0500
     >I  have  yet  to  run  a 34BE12 stain on either a prostate bx or a
     prostate big that was completely negative for the stain.
     >
     >Glen Dawson
     >
     >-----Original Message-----
     >From: histonet-bounces <@t> lists.utsouthwestern.edu
     >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On   Behalf   Of
     Jennifer
     >MacDonald
     >Sent: Tuesday, May 16, 2006 11:08 AM
     >To: Roxanne Soto
     >Cc: histonet-bounces <@t> lists.utsouthwestern.edu;
     >HISTONET <@t> PATHOLOGY.SWMED.EDU
     >Subject: Re: [Histonet] 34BE12
     >
     >
     >What if there is no "normal" tissue?
     >
     >
     >
     >
     >
     >
     >
     >
     >"Roxanne Soto" <godsgirlnow <@t> msn.com>
     >Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
     >05/16/2006 09:39 AM
     >
     >To
     >HISTONET <@t> PATHOLOGY.SWMED.EDU
     >cc
     >
     >Subject
     >[Histonet] 34BE12
     >
     >
     >
     >
     >
     >
     >
     >    Gotta question for you ...
     >    how  many  of  you  out  there  are  running  a positive and a
     negative
     >    control  for  your   34be12   on   prostates?  How   many  are
     not?  How do you
     >    handle  this  in  your  procedure  under your QC?  I have been
     asked to do
     >    both.    Some  want  a  control  and  some  don't  I am trying
     to get a
     >    concensus and be uniform.
     >    The argument for no control.....it stains for normal tissue
     >    The arguement for a control....it still stains......
     >
     >    Any suggestions?
     >    Roxanne
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