[Histonet] Skin Artifact Identification

[Rene J Buesa]

Santoianni & Hammami published an article in Jorunal Histotechnol.[13(2):135-136(1990)] where they attribute a nuclear "bubbling" artifact to oven heat-affixing the sections above 60ºC before they were completely free from water underneath.
  I have been able to corroborate their finding. If this makes sense to you try to be absolutely sure that there is no water left underneath your sections before heating them in the oven.
  Hope this will help you!
  René J.

JGREWE <@t> OhioHealth.com wrote:
  Does anyone know what may be causing this skin artifact? What we have in 
some of our skin cases is vacuoles within the nuclei of squamous cells of 
epidermis on permanent sections. We don't see it all of the time. 10% 
NBF fixation. Thanks
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