[Histonet] Loss of immunoreactivity in cryosections

Johnson, Teri TJJ <@t> Stowers-Institute.org
Mon May 15 10:27:31 CDT 2006


Has anyone experienced a loss of immunoreactivity in cryosections that
have airdried overnight prior to staining or storage at -80 degrees C?
And if so, are these unfixed cryosections, or fixed, cryoprotected
cryosections? Thank you for any information you can provide, the more
info, the better.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133



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