[Histonet] Blocking endogenous peroxidase in pig LN

[Laurie Reilly]

Histonetters,
Has anyone had experience with IHC on pig (hog) tissues?
We are having trouble trying to block endogenous peroxidase in eosinophils 
in frozen sections of lymph nodes.
We have tried hydrogen peroxide up to 6% for one hour.
Periodic acid at 0.23% for 1 minute helped but didn't remove all the 
staining, longer time (up to 8 minutes) didn't improve the removal.

Any help would be greatly appreciated.

    Regards,      Laurie.



Mr.Laurie Reilly
School of Veterinary and Biomedical Sciences
James Cook University
Townsville. 4811
Australia.

Phone  07 4781 4468
Fax      07 4779 1526