[Histonet] IHC on Sakura Xpress processed tissue

[Bartlett, Jeanine (CDC/NCID/VR)]

I had the pathologists routinely gross two sets of tissues for me (when
there was enough tissue), one for traditional processing and the other
for the Xpress.  IHC testing on the tissues processed by both methods
was run in the same experiment.  In the beginning, the controls we used
were not microwave processed but if the diagnostic cases came back
positive by both routine and microwave processing then it was apparent
that there was not a problem.  We have since be working on cell culture
controls and testing them to see how the microwave processing affects
them.  We make ours in Histogel and since cell controls require less
time in the Xpress pre-processing solution I have trying to get that
optimized to avoid over-hardening.


Jeanine Bartlett, BS, HT(ASCP)
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Janci
Wellborn
Sent: Friday, May 12, 2006 9:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue

How did you do the compairson for IHC control tissue?  Don't the control
tissues have to be treated the same as the patients?  When you switched
from regular processing to microwave, did you do any compairsons between
the two forms of processing?

Janci Wellborn, BS, BSeD, HTL (ASCP)
Dunes Medical Laboratory
Dakota Dunes, SD
wellborj <@t> mercyhealth.com 


>>> "Bartlett, Jeanine (CDC/NCID/VR)" <jqb7 <@t> cdc.gov> 5/11/2006 1:24 PM
>>>
For us there has been no problem at all.  In some cases the IHC staining
is actually crisper.  I will say that we primarily have formalin-fixed
tissues (we receive most of our cases from outside CDC).  


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, May 11, 2006 2:14 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] IHC on Sakura Xpress processed tissue

Can I get feedback from the Histonet world on how IHC staining is
effected by switching from standard processing to processing on the
Xpress?
Does anyone see any other drawbacks to the technology?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916



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