[Histonet] IHC on Sakura Xpress processed tissue

Janci Wellborn wellborj <@t> mercyhealth.com
Fri May 12 08:01:15 CDT 2006


How did you do the compairson for IHC control tissue?  Don't the control
tissues have to be treated the same as the patients?  When you switched
from regular processing to microwave, did you do any compairsons between
the two forms of processing?

Janci Wellborn, BS, BSeD, HTL (ASCP)
Dunes Medical Laboratory
Dakota Dunes, SD
wellborj <@t> mercyhealth.com 


>>> "Bartlett, Jeanine (CDC/NCID/VR)" <jqb7 <@t> cdc.gov> 5/11/2006 1:24 PM
>>>
For us there has been no problem at all.  In some cases the IHC
staining
is actually crisper.  I will say that we primarily have formalin-fixed
tissues (we receive most of our cases from outside CDC).  


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, May 11, 2006 2:14 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] IHC on Sakura Xpress processed tissue

Can I get feedback from the Histonet world on how IHC staining is
effected by switching from standard processing to processing on the
Xpress?
Does anyone see any other drawbacks to the technology?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916



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