[Histonet] RE: Histonet Digest, Vol 30, Issue 15
Rice, Michael
Michael.Rice <@t> holy-cross.com
Thu May 11 12:46:24 CDT 2006
We have been using eosin in the 95% station on our processors for years and have never had a problem. It is a life server for all of the smaller and smaller biopsies that we are receiving these days in addition to dealing with older techs like me with failing eyesight. It is the perfect storm.
Mike
Michael Rice CT.HT(ASCP)
Supervisor Of Pathology
Holy Cross Hospital
Ft Lauderdale, Fl 33308
954.776.3070
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, May 11, 2006 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 30, Issue 15
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: double immunostaining (Johnson, Teri)
2. Marking prostate biopsies (Vickroy, Jim)
3. Anatech Hp Stain (Parker, Helayne)
4. RE: Marking prostate biopsies (Kemlo Rogerson)
5. RE: Marking prostate biopsies (Gladney, Diane C Ms MACH)
6. RE: Immuno. for Cat Scratch (Monfils, Paul)
7. Re: Marking prostate biopsies (Fred Underwood)
8. Cryopreservation dry ice vs LN2 isopentane (Jamie E Erickson)
9. Re: safranin o staining- glycosaminoglycans-cartilage
(Gayle Callis)
10. RE: Marking prostate biopsies (hymclab)
11. Re: Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech)
(John A. Kiernan)
12. STAFF EVALUATIONS (Diana McCaig)
13. RE: Immuno. for Cat Scratch (Bales, Candy A)
14. RE: Marking prostate biopsies (Temple Nancy)
15. Re: STAFF EVALUATIONS (Rene J Buesa)
16. Re: Cryopreservation dry ice vs LN2 isopentane (Gayle Callis)
17. autofluorescence? (yasushi nakagawa)
18. Re: Marking prostate biopsies (Lori Richey)
----------------------------------------------------------------------
Message: 1
Date: Thu, 11 May 2006 09:17:12 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: double immunostaining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E78044B7D3F <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Dear Richard,
Kits are not needed to design and successfully do doublestaining. I
recommend you buy Chris van der Loos' book on the subject, and therein
lies pretty much all the information you will need. ISBN: 038791594X
You should be able to get this through Amazon.com and other online book
vendors.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133
------------------------------
Message: 2
Date: Thu, 11 May 2006 09:28:50 -0500
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] Marking prostate biopsies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A2AD4F58AD06B44FBA71D4B1C4B9571A0680A26F <@t> mmcmail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
We are looking for a way to better mark our small needle biopsies such
as breast cores and prostate biopsies so that they are more easily seen
after processing.
Right now the embedder has some trouble identifying the cores and in
turn the histotech often has problems seeing the cores in the paraffin
block.
We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.
I have heard of some techs who have used a little eosin in their 70%
ETOH, which helped the techs identify the cores. I am reluctant to use
eosin on my automated processors in the 70% ETOH however. We tried
dipping the blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good. Does anyone have any good
ideas?
I do know that some send eosin tinted formalin to sites that are doing
these biopsies. I don't see that as an option for us. Thanks for your
help.
James R. Vickroy
Supervisor - MMC Surgical Pathology
217-788-4046
This message (including any attachments) contains confidential information intended for a
specific individual and purpose, and is protected by law. If you are not the intended recipient,
you should delete this message. Any disclosure, copying, or distribution of this message, or the
taking of any action based on it, is strictly prohibited.
------------------------------
Message: 3
Date: Thu, 11 May 2006 09:31:59 -0500
From: "Parker, Helayne" <HParker <@t> Skaggs.Net>
Subject: [Histonet] Anatech Hp Stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2FABC6145388F24EBA8CBD6EC165BCCB02AFBA69 <@t> mail1-schc.skaggs.net>
Content-Type: text/plain; charset="US-ASCII"
Hi All,
We have been having trouble with the Hp stain. We get green instead
of bright yellow. Depending on the green we can easily see the bacteria
and other times not. Dr. is complaining as very inconsistent. We have
tried the product insert suggestion to no great avail.
Any suggestions,
Helayne Parker, HT (A.S.C.P.)
Histology Section Head
Skaggs Community Health Center
Branson, Missouri
------------------------------
Message: 4
Date: Thu, 11 May 2006 15:36:35 +0100
From: Kemlo Rogerson <kemlo.rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Marking prostate biopsies
To: "'Vickroy, Jim'" <Vickroy.Jim <@t> mhsil.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B4FA3DD12D42DA11A5DA00508BAF864902FDFE0F <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain
I used to dip breast biopsy cores into very dilute alcoholic eosin just for
a few seconds and they came through the Processors OK. You could post fix
after formalin with a picric acid fixative but then you'd have to wash it
out.
If you decide to use eosin then don't use a single receptacle for dipping
the biopsies into; cross over. I used to put a few drops into the upturned
lid of the biopsy pot and then dip the cores in, one by one, then put into
the cassette. I used then to forget and put the lid on the pot and cover
myself in eosin, and the floor, and my coat, and the MLA next to me. But
better that then give someone a false positive result.
A bit red in the face.....
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
The real does not die, the unreal never lived. --Nisargadatta Maharaj
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your
co-operation
------------------------------
Message: 5
Date: Thu, 11 May 2006 10:36:02 -0400
From: "Gladney, Diane C Ms MACH" <Diane.Gladney <@t> se.amedd.army.mil>
Subject: RE: [Histonet] Marking prostate biopsies
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302DE91FD <@t> amedmlsermc133>
Content-Type: text/plain; charset="us-ascii"
I use Eosin in my last 100% Ethanol on my tissue processor. It works
great for the small biopsies of other tissue also. We have not had any
problems seeing these small biopsies or needle core biopsies since I
started doing this.
Diane
Diane C. Gladney, HT (ASCP)
Supervisor, Histology Dept.
Moncrief Army Community Hospital
Dept. of Pathology
4500 Stuart St.
FT. Jackson, SC 29207
Email: diane.gladney <@t> se.amedd.army.mil
Phone: 803-751-2530
FAX: 803-751-7829
DSN: 734-2530
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Thursday, May 11, 2006 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Marking prostate biopsies
We are looking for a way to better mark our small needle biopsies such
as breast cores and prostate biopsies so that they are more easily seen
after processing.
Right now the embedder has some trouble identifying the cores and in
turn the histotech often has problems seeing the cores in the paraffin
block.
We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.
I have heard of some techs who have used a little eosin in their 70%
ETOH, which helped the techs identify the cores. I am reluctant to use
eosin on my automated processors in the 70% ETOH however. We tried
dipping the blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good. Does anyone have any good
ideas?
I do know that some send eosin tinted formalin to sites that are doing
these biopsies. I don't see that as an option for us. Thanks for your
help.
James R. Vickroy
Supervisor - MMC Surgical Pathology
217-788-4046
This message (including any attachments) contains confidential
information intended for a
specific individual and purpose, and is protected by law. If you are not
the intended recipient,
you should delete this message. Any disclosure, copying, or distribution
of this message, or the
taking of any action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 11 May 2006 10:41:16 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Immuno. for Cat Scratch
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017176F7 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Antibody against Bartonella henselae, the agent of cat scratch disease,
exists, because some commercial labs offer the immunofluorescence technique.
However, I don't know of a source for the antibody.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Marian Powers
> Sent: Wednesday, May 10, 2006 5:47 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Immuno. for Cat Scratch
>
> Wondering if there is an antibody for Cat Scratch Bacillus?
>
> Thanks in advance,
>
> Marian
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 7
Date: Thu, 11 May 2006 10:51:29 -0400
From: "Fred Underwood" <funderwood <@t> mcohio.org>
Subject: Re: [Histonet] Marking prostate biopsies
To: <histonet <@t> lists.utsouthwestern.edu>, <Vickroy.Jim <@t> mhsil.com>
Message-ID: <s4631738.071 <@t> mcohio.org>
Content-Type: text/plain; charset=US-ASCII
I have used Mrs. Stewarts Bluing Agent. It's a product used for
whitening clothes. We would dip prostate biopsies, or place a drop onto
the biopsy, at the grossing table. Blot off any excess,wrap in lens
paper, and process. The biopsies would be colored a highly visible
blue.
Fred
>>> "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com> 05/11/06 10:28AM >>>
We are looking for a way to better mark our small needle biopsies such
as breast cores and prostate biopsies so that they are more easily
seen
after processing.
Right now the embedder has some trouble identifying the cores and in
turn the histotech often has problems seeing the cores in the paraffin
block.
We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.
I have heard of some techs who have used a little eosin in their 70%
ETOH, which helped the techs identify the cores. I am reluctant to
use
eosin on my automated processors in the 70% ETOH however. We tried
dipping the blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good. Does anyone have any good
ideas?
I do know that some send eosin tinted formalin to sites that are doing
these biopsies. I don't see that as an option for us. Thanks for
your
help.
James R. Vickroy
Supervisor - MMC Surgical Pathology
217-788-4046
This message (including any attachments) contains confidential
information intended for a
specific individual and purpose, and is protected by law. If you are
not the intended recipient,
you should delete this message. Any disclosure, copying, or
distribution of this message, or the
taking of any action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 11 May 2006 11:03:24 -0400
From: Jamie E Erickson <jamie.erickson <@t> abbott.com>
Subject: [Histonet] Cryopreservation dry ice vs LN2 isopentane
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF65327026.FDA55BB5-ON8525716B.0050EE0E-8525716B.0052B58E <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Hello histonetters,
I am hoping that someone out there
can help me with a cryopreservation dilemma. I am in a research pathology
lab and we have two schools of cryopreservation here, some researchers use
dry ice isopentane and others use liquid nitrogen isopentane. I have heard
that LN2 is faster with less ice crystal formation and I think this is a
better way to freeze but I need to convince the dry ice people to use LN2.
Does anyone have papers that will support my fight for one standard way of
freezing. Any thoughts or help with literature would be great. We freezing
mouse and rat tissues for IHC and LCM (laser capture microdissection ).
Thanks
_______________________________
Jamie Erickson
Sr. Research Associate
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
------------------------------
Message: 9
Date: Thu, 11 May 2006 09:12:29 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] safranin o staining-
glycosaminoglycans-cartilage
To: PKamalavenkatesh <@t> wockhardtin.com,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060511090752.01b1a650 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I will be attaching the safranin O/fast green method to you privately, the
original publication was by Rosenberg, reference will be in
protocol. Rosenberg originally did this on frozen sections of articular
cartilage and it has since been modified for formalin fixed, decalcified
bone paraffin sections. It is wise to include an undecalcified, normal
piece of articular cartilage as a normal control to see the tinctorial
quality compared to a decalcified cartilage to ensure decalcifiers have not
affected the glycoaminoglycans.
At 05:22 AM 5/11/2006, you wrote:
>Dear Histonetters,
> I am in need of the procedure for staining of
>glycosaminoglycans in articular cartilage. The literature search indicated
>the use of safranin o staining for the characterization of
>glycosaminoglycans. If any body have good experience in this procedure and
>able to share their protocol. I have already ordered a paper that deals
>with this.
>Method of Histomorphometric Assessment of Glycosaminoglycans in Articular
>Cartilage
>Journal of Orthopaedic Research Vol 15, 670-674. 1977
>Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke
>Hirasawa and David Amiel.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 10
Date: Thu, 11 May 2006 10:19:10 -0500
From: hymclab <hymclab <@t> hyhc.com>
Subject: RE: [Histonet] Marking prostate biopsies
To: "'Vickroy, Jim'" <Vickroy.Jim <@t> mhsil.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B1D0FAA1DF69EA4D82E274131A89BC222BFD82 <@t> mhcchyhcexchange.ministryhealth.net>
Content-Type: text/plain
We put hematoxylin on our cores at the grossing station. We pull out the
cores, place them on a towel and use a pipette to place a drop of
hematoxylin on each core. The cores are them placed between blue sponges
that have been soaked in formalin and them processed. The cores are a nice
purple and show up well while embedding and while cutting and of course
doesn't interfere with staining.
Hope this helps,
Dawn
-----Original Message-----
From: Vickroy, Jim [mailto:Vickroy.Jim <@t> mhsil.com]
Sent: Thursday, May 11, 2006 9:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Marking prostate biopsies
We are looking for a way to better mark our small needle biopsies such as
breast cores and prostate biopsies so that they are more easily seen after
processing.
Right now the embedder has some trouble identifying the cores and in turn
the histotech often has problems seeing the cores in the paraffin block.
We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.
I have heard of some techs who have used a little eosin in their 70% ETOH,
which helped the techs identify the cores. I am reluctant to use eosin on
my automated processors in the 70% ETOH however. We tried dipping the
blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good. Does anyone have any good
ideas?
I do know that some send eosin tinted formalin to sites that are doing these
biopsies. I don't see that as an option for us. Thanks for your help.
James R. Vickroy
Supervisor - MMC Surgical Pathology
217-788-4046
This message (including any attachments) contains confidential information
intended for a specific individual and purpose, and is protected by law. If
you are not the intended recipient, you should delete this message. Any
disclosure, copying, or distribution of this message, or the taking of any
action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 11 May 2006 11:14:27 -0400
From: "John A. Kiernan" <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FD
Neurotech)
To: John Fernandez <jfern <@t> unimelb.edu.au>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <446354D3.D15CBAB3 <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii
The first thing you need to find out is whst's in
the kit.
Is the method the one traditionally known as the
rapid Golgi method? This involves fixation in an
osmium tetroxide-potassium dichromate mixture, for
2 to 7 days (the time affects the outcome, but
unpredictably) followed by immersion in aqueous
silver nitrate for a few days, and preparing thick
celloidin or paraffin sections. There are plenty
of later variants, having in common a silver
chromate end product, but these should not be
called "the rapid Golgi method". The sections are
mounted in thick Canada balsam (the real stuff)
without coverslips. If a coverslip is applied, the
preparations fade with time. There are various
chemical tricks to prevent such fading.
The other major member of this group of methods is
Cox's, with mercuric chloride and potassium
dichromate, but no silver. The dark deposit in
this case is thought to be a mixture of mercurous
oxide and colloidal mercury. These methods are
often called Golgi-Cox, even though Golgi didn't
invent or use them. Ideally Golgi-Cox preparations
should also be mounted without coverslips, but
they will keep for a few years in DPX with
coverslips.
John Kiernan
Anatomy, UWO
London, Canada
____________________
John Fernandez wrote:
>
> Hi all,
>
> our lab has recently purchased and used the Rapid Golgi Stain Kit from FD
> Neurotech, resulting in very impressive staining down to dendritic spine
> level, however as Julie Heinrich found, within 24 hours this staining
> becomes very granular with loss of distinct morphology that was otherwise
> seen previously. It progressively worsens over time.
>
> Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does
> anyone know of a mounting medium that can be used to adequately stop the
> reaction that is taking place? Any ideas on what could be causing this
> progressive deterioration of staining would be very much appreciated.
>
> Thanks in advance,
>
> John
>
> John Fernandez
> Research Assistant
> Department of Medicine
> University of Melbourne
> Austin & Repatriation Medical Centre
> Heidelberg, VIC 3084
> Ph: (03) 9496 3257
>
> Re: Golgi stain question
> From: "J. A. Kiernan" <jkiernan <@t> uwo.ca>
>
> --------------------------------------------------------------------------------
>
> On Fri, 30 Mar 2001, Julie Heinrich wrote:
>
> > I am terribly sorry to bother you- I have been looking through the
> > histonet web page and have found several helpful replies from you
> > about the Gibb & Kolb Golgi stain method. I haven't managed to
> > figure out how to properly post a question there -
>
> You can't. It's a collection of old (archived) communications.
> To ask or answer questions you have to subscribe to the
> listserver. This is done by sending an email to
> histonet <@t> pathology.swmed.edu with the one word subscribe
> in the Subject line. Nothing else. You'll then get an automatic
> reply from the listserver telling you all about it.
>
> > I'm attempting to use the method on avian tissue. I have obtained
> > some decent looking tissue so far (though the stain is a dark
> > tan/brown, rather than the preferable black that I had expected) yet
> > after time the stain turns very 'grainy'. Within a matter of just 24
> > hours, the dendrites/spines look like collections of dots rather than
> > complete structures, and it gets worse with time.
>
> > Do you have any idea why this might be happening? (I'm following
> > their protocol to the best of my knowledge, and use fresh solutions
> > each time I run tissue)
>
> A graduate student here called Tim Ho did great numbers of Kolb
> Golgis on rat brains a few years ago. He went to Kolb's lab in
> Lethbridge, Alta for guidance. His initial problem was that the
> unstained spaces between the black cells were pale green and
> a bit granular. If I remember rightly, the washing after the
> Golgi-Cox solution needed to be more thorough.
>
> Your problem is different, and may relate to the mounting medium.
> Traditionally the sections were mounted in thick Canada balsam
> without coverslips. A modern synthetic medium + coverslip can
> be followed by fading, but I haven't heard of this happening
> in 24 hours. 6 months, yes. Are you cutting vibratome sections
> of adequate thickness? I think you must be doing something wrong
> at or after the sectioning step, but don't know what. Sorry I
> can't be more helpful.
>
> ----------------------------------------
> John A. Kiernan
> Department of Anatomy & Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan <@t> uwo.ca
> http://publish.uwo.ca/~jkiernan
>
> --------------------------------------------------------------------------------
>
> << Previous Message | Next Message >>
> --
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Thu, 11 May 2006 11:13:18 -0400
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] STAFF EVALUATIONS
To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCFD9E6A390E294AAF3A2561CD32E5C40DC29D <@t> ckhamail1.ckha.on.ca>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone do an annual evaluation of staff ability to perform certain tasks?
Diana McCaig 519-352-6401 (6604)
------------------------------
Message: 13
Date: Thu, 11 May 2006 10:22:25 -0500
From: "Bales, Candy A" <candy.a.bales <@t> uth.tmc.edu>
Subject: RE: [Histonet] Immuno. for Cat Scratch
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EA1FDD2A141B7448B4B1AFFFCAC08DE4065865EB <@t> UTHEVS1.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Biocare Medical sells one for Cat Scratch (Bartonella henselae). Source:
Mouse.
Have not tried products with this company yet, just met local rep. &
obtained a catalog. Their phone # 800-799-9499 or www.biocare.net
C. Bales
UT-Houston
-----Original Message-----
>
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 11 May 2006 11:27:58 -0400
From: "Temple Nancy" <Nancy.Temple <@t> ssfhs.org>
Subject: RE: [Histonet] Marking prostate biopsies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FB239D467A25DE40A08FADAA3192FD0201749444 <@t> oaintsedb1.ssfhs.org>
Content-Type: text/plain; charset="us-ascii"
We also put Eosin in our last 100% alcohol on the processor. Helps with
embedding and especially with cutting of needle bx and other small bx.
Our bx are between blue sponges(I like the sponges from SurgiPath
best)that have been soaked in formalin.
Nancy
St. Francis Hospital
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Thursday, May 11, 2006 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Marking prostate biopsies
We are looking for a way to better mark our small needle biopsies such
as breast cores and prostate biopsies so that they are more easily seen
after processing.
Right now the embedder has some trouble identifying the cores and in
turn the histotech often has problems seeing the cores in the paraffin
block.
We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.
I have heard of some techs who have used a little eosin in their 70%
ETOH, which helped the techs identify the cores. I am reluctant to use
eosin on my automated processors in the 70% ETOH however. We tried
dipping the blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good. Does anyone have any good
ideas?
I do know that some send eosin tinted formalin to sites that are doing
these biopsies. I don't see that as an option for us. Thanks for your
help.
James R. Vickroy
Supervisor - MMC Surgical Pathology
217-788-4046
This message (including any attachments) contains confidential
information intended for a specific individual and purpose, and is
protected by law. If you are not the intended recipient, you should
delete this message. Any disclosure, copying, or distribution of this
message, or the taking of any action based on it, is strictly
prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 11 May 2006 08:44:03 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] STAFF EVALUATIONS
To: Diana McCaig <dmccaig <@t> ckha.on.ca>, "Histonet \(E-mail\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20060511154403.18424.qmail <@t> web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Once a year to all staff member and tasks. During probation there were monthly evaluations.
René J.
Diana McCaig <dmccaig <@t> ckha.on.ca> wrote:
Does anyone do an annual evaluation of staff ability to perform certain tasks?
Diana McCaig 519-352-6401 (6604)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2¢/min with Yahoo! Messenger with Voice.
------------------------------
Message: 16
Date: Thu, 11 May 2006 09:56:47 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Cryopreservation dry ice vs LN2 isopentane
To: Jamie E Erickson <jamie.erickson <@t> abbott.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060511094620.01b7e320 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Jamie,
Using dry ice mixed with isopentane or hexane will work just as well for
snap freezing (i.e. cryopreservation?) as liquid nitrogen/isopentane.
The problem with liquid nitrogen and isopentane is the constant recooling
of the isopentane to the correct temperature when snap freezing a large
number of tissues during a long freezing session We gave up on this
method years ago and opted for dry ice/isopentane or hexane slurry. You
can also use a petri dish floating in liquid nitrogen, that way you can
snap freeze multiple samples rapidly, and get rid of isopentane, always a
storage dilemma unless you have explosion proof freezers/refrigerators. We
often snap freeze 30 blocks during a dissection/snap freezing session. We
also had our OCT crack in the liq N2/isopentane method, it was just too cold.
I will be happy to send you a powerpoint showing how this is done (both
methods). We do not have freezing artifact.
For LCM, a colleague and expert at doing this, snap freezes tissues in dry
ice cooled hexane but does NOT put the dry ice into the solvent, she merely
surrounds the beaker of hexane with dry ice until correct temperature is
needed. You can discuss this with her via email privately, I will send her
email address to you privately if you want it. She does work with rodent
tissues.
No papers, just experience and learning the hard way for our
laboratory. MyNeuroLab website has a discussion or at least Charles
Scouten on the theory of snap freezing.
At 09:03 AM 5/11/2006, you wrote:
>Hello histonetters,
> I am hoping that someone out there
>can help me with a cryopreservation dilemma. I am in a research pathology
>lab and we have two schools of cryopreservation here, some researchers use
>dry ice isopentane and others use liquid nitrogen isopentane. I have heard
>that LN2 is faster with less ice crystal formation and I think this is a
>better way to freeze but I need to convince the dry ice people to use LN2.
>Does anyone have papers that will support my fight for one standard way of
>freezing. Any thoughts or help with literature would be great. We freezing
>mouse and rat tissues for IHC and LCM (laser capture microdissection ).
>
>Thanks
>
>
>_______________________________
>Jamie Erickson
>Sr. Research Associate
>Department: DSMP
>Abbott Bioresearch Center
>100 Research Drive
>Worcester, MA 01605-4341
>508-688-3134
>FAX: 508-793-4895
>e-mail: jamie.erickson <@t> abbott.com
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 17
Date: Thu, 11 May 2006 11:12:51 -0500
From: yasushi nakagawa <nakagawa <@t> umn.edu>
Subject: [Histonet] autofluorescence?
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <C75EDC93-ACD7-4EEB-BB23-AA954255A498 <@t> umn.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Hi,
We are doing immunohistochemistry using Cy2, Cy3 and Cy5-conjugated
secondary antibodies on 20um-thick cryosections of embryonic mouse
brains. Especially with Cy2- filter setting, we sometimes see a
number of auto-fluorescence-like signals within the brain sections.
They are thin and long, kind of looking like blood vessels. They show
up even when we do not use Cy2-conjugated secondaries. The same
problem has happened to Cy3-filter setting, too, although less
frequently. I wonder if anyone has a idea about what they are and how
to prevent them from showing up.
We fix brains in 4%PFA/PB overnight, wash and cryoprotect in sucrose/
PB, and freeze in OCT in plastic molds on petri dish on liquid
nitrogen. We cut 20um cryosections, dry them for an hour or so, and
do regular antibody staining, do DAPI staining, dehydrate, and mount
in DPX.
Thank you for your help.
Yasushi Nakagawa
------------------------------
Message: 18
Date: Thu, 11 May 2006 09:20:03 -0700
From: Lori Richey <lrichey <@t> u.washington.edu>
Subject: Re: [Histonet] Marking prostate biopsies
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <44636433.8040800 <@t> u.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Mercurichrome works well for cardiac bxs. Not sure what percent.
Vickroy, Jim wrote:
>
>
>We are looking for a way to better mark our small needle biopsies such
>as breast cores and prostate biopsies so that they are more easily seen
>after processing.
>
>Right now the embedder has some trouble identifying the cores and in
>turn the histotech often has problems seeing the cores in the paraffin
>block.
>
>We are doing levels on these cases but find that often we need recuts
>because we are not into the block enough.
>
>
>
>I have heard of some techs who have used a little eosin in their 70%
>ETOH, which helped the techs identify the cores. I am reluctant to use
>eosin on my automated processors in the 70% ETOH however. We tried
>dipping the blocks in eosin tinted formalin at the grossing stations.
>The eosin leached out and did no good. Does anyone have any good
>ideas?
>
>
>
>I do know that some send eosin tinted formalin to sites that are doing
>these biopsies. I don't see that as an option for us. Thanks for your
>help.
>
>
>
>James R. Vickroy
>
>Supervisor - MMC Surgical Pathology
>
>217-788-4046
>
>
>
>
>
>This message (including any attachments) contains confidential information intended for a
>specific individual and purpose, and is protected by law. If you are not the intended recipient,
>you should delete this message. Any disclosure, copying, or distribution of this message, or the
>taking of any action based on it, is strictly prohibited.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 30, Issue 15
****************************************
======================
Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original.
======================
More information about the Histonet
mailing list