[Histonet] Re: Histonet Digest, Vol 30, Issue 8

Sun May 7 22:03:17 CDT 2006

Hi everyone
  Can anyone recommend anti MMP-1, 2 and 9 antibodies that work well on formalin fixed paraffin embedded tissues?
  Thanks
  John

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Today's Topics:

1. Job Opening (Luck, Greg D.)
2. Re: Microwave and DNA/Molecular (Johnson, Teri)
3. FW: MyNeuroLab.com Media (Charles Scouten)
4. Re: FW: H&E on Leica XL automatic stainer
(mari.ann.mailhiot <@t> leica-microsystems.com)
5. Re: counter-top tissue processors (Andrea Hooper)
6. Marker for invasive carcinoma (Jeff Silverman)
7. Re: texture/quality of DAB staining (Andrea Hooper)
8. Pancreas markers (Jeff Silverman)

----------------------------------------------------------------------

Message: 1
Date: Fri, 5 May 2006 10:22:19 -0700
From: "Luck, Greg D." 
Subject: [Histonet] Job Opening
To: 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello all,

We have an histology position open for an HT or HTL certifed tech.
Routine histology duties (embedding, cutting and special stains. IHC
experience in addition preferred. This position is in a 300 bed
community hospital in Spokane, WA.
Below I have placed three weblinks for those interested so that they can
go on the internet to look at our facility, corporate organization and
the community and surrounding region. A great place to work and live.
Please contact me directly.

Thanks, Greg

www.empirehealth.org www.deaconessmc.org
www.visitspokane.com

Greg Luck
Anatomic Path Spvr
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org 

------------------------------

Message: 2
Date: Fri, 5 May 2006 13:13:59 -0500
From: "Johnson, Teri" 
Subject: [Histonet] Re: Microwave and DNA/Molecular
To: 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Doug,

I think the jury is still out on that. Microwave radiation is
non-ionizing electromagnetic energy similar to radar and sound waves.
There have been some reports of state troopers getting tumors due to the
radar guns, or folks having tumors from using cell phones. I don't know
how good the science is behind it, but I'm thinking you'll find reports
which state they do cause damage, and others which state they don't.

If you're talking about what a microwave tissue processor would do to
your DNA in the sample, I believe the radiation would not harm it. The
molecules will vibrate in response to exposure to the microwaves and
that is what generates the heat. Keeping the temperature controlled
should keep the DNA from denaturing. High temperatures in the microwave,
as those achieved doing HIER techniques, will surely cause DNA strands
to separate. However, I suspect cooling should allow them to pair back
up. But for routine processing, the heat never gets that high.

I found information on this website
(http://www.gallawa.com/microtech/mwave.html) which doesn't directly
answer your question, but it does provide good information. Kok and Boon
also have a book on Microwave technology you can buy through Milestone
Medical.

Good luck,

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

------------------------------

Message: 3
Date: Fri, 5 May 2006 14:41:44 -0500
From: "Charles Scouten" 
Subject: [Histonet] FW: MyNeuroLab.com Media
To: 
Message-ID:
<5784D843593D874C93E9BADCB87342AB01306EE3 <@t> tpiserver03.Coretech-holdings.com>

Content-Type: text/plain; charset="us-ascii"

It sounds like she is making a Ralph knife for sectioning
GMA/JB-4/HistOresin. Yes, these knife makers are still available. Glass
knives are used extensively in EM labs but made in a different
configuration, a triangled knife. It sounds like the knife breaker is in
bad need of service. The knife scoreing wheel probably needs to be
replaced, adjusted along with a few other replaceable parts. What model
of glass breaker are you using?

Lee

Lee E. Dickey

Product Marketing and Sales Manager

McCormick Scientific

________________________________

From: Tabitha Granshaw [mailto:TGranshaw <@t> biocompare.com] 
Sent: Wednesday, May 03, 2006 5:05 PM
To: Charles Scouten
Cc: Marla Steuer
Subject: RE: MyNeuroLab.com Media

Hi Charles,

I just wanted to drop you a line to see if MyNeuroLab would be
interested in a Promotion. This Promotion would be covered by the
contract so there would be no additional charge.

The Promotions can be viewed on the Biocompare site at
http://www.biocompare.com/contents/9/Promotions-and-Special-Offers.html

If you would be interested in setting one up, don't hesitate to drop me
a line

Cheers,
Tabitha

________________________________

From: Charles Scouten [mailto:cwscouten <@t> myneurolab.com] 
Sent: Friday, March 31, 2006 9:55 AM
To: Tabitha Granshaw
Subject: RE: MyNeuroLab.com Media

Thanks, thats fine.

Cordially,

Charles W. Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX 314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 

________________________________

From: Tabitha Granshaw [mailto:TGranshaw <@t> biocompare.com] 
Sent: Friday, March 31, 2006 11:08 AM
To: Charles Scouten
Cc: Marla Steuer
Subject: RE: MyNeuroLab.com Media

Good Morning Charles,

The two sponsorship placements would be covered by the current contract,
so there would be no additional charge. 

Since it sounds like the sponsorships are okay with you, I've gone ahead
and scheduled them for:

April 20
May 25

As the text I sent to you yesterday looks okay, I'll go ahead and place
that in the two sponsorship spots.

Drop me a line if you have any questions

Cheers,
Tabitha

Tabitha Granshaw
Manager of Media Coordination
Biocompare
The Buyer's Guide for Life Scientists
395 Oyster Point Blvd. Suite 405
South San Francisco, CA 94080
Phone (650) 416-0505
http://www.biocompare.com

________________________________

From: Charles Scouten [mailto:cwscouten <@t> myneurolab.com] 
Sent: Friday, March 31, 2006 7:31 AM
To: Tabitha Granshaw
Subject: RE: MyNeuroLab.com Media

yes, the attached text would be fine. I am a little confused, am I
hereby authorizing a funds expenditure? I have not previosuly been in
on what we do with these, and know we are short of cash these day.
Sponsorship sounds like if costs us money. How much?

Cordially,

Charles W. Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX 314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 

________________________________

From: Tabitha Granshaw [mailto:TGranshaw <@t> biocompare.com] 
Sent: Thursday, March 30, 2006 6:33 PM
To: Charles Scouten
Cc: Marla Steuer
Subject: MyNeuroLab.com Media

Hi Charles,

Thanks for sending over the text for the New Technology - I've started
the postig process for that and will let you know when it's live.

Also, I'd like to schedule a couple Neuroscience sponsorships for
MyNeuroLab. If you like we could schedule April 20 for MyNeuroLab.com,
and perhaps also a date in May. Would the attached text be okay to run?
(It's the text that was used for the most recent MyNeuroLab.com
sponsorship)

If you could let me know about this soon, that would be great as these
spots go quickly

Thanks

Tabitha

Tabitha Granshaw
Manager of Media Coordination
Biocompare
The Buyer's Guide for Life Scientists
395 Oyster Point Blvd. Suite 405
South San Francisco, CA 94080
Phone (650) 416-0505
http://www.biocompare.com

------------------------------

Message: 4
Date: Fri, 5 May 2006 14:49:36 -0500
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] FW: H&E on Leica XL automatic stainer
To: "Sanders, Julie, VHACIN" 
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=US-ASCII

Hi Julie

I will be happy to send you some protocols for the AutoXL if you would
like.

Please let me know

Best Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com

"Sanders, Julie, 
VHACIN" 
.gov> 
Sent by: cc 
histonet-bounces@ 
lists.utsouthwest Subject 
ern.edu [Histonet] FW: H&E on Leica XL 
automatic stainer 

05/05/2006 11:21 
AM 

-----Original Message-----
From: Sanders, Julie, VHACIN
Sent: Friday, May 05, 2006 11:28 AM
To: histonet <@t> lists.utsouthwestern.ed; 200/316 Unmatched Invoices
Subject: H&E on Leica XL automatic stainer

Hi Netters,
I am trying to find different protocols for H&E on the Leica autostainer
XL. I would appreciate anyone using this stainer sharing their procedure.
Currently we are using Gills lll and Richard Allen Eosin...but I am
interested in any/all procedures. Feel free to email them to me privately
if you wish.
Thanks in advance,
Julie

Julie A. Sanders, BA,HT(ASCP)
Supervisor, Anatomic Pathology
Cincinnati VAMC
Julie.Sanders <@t> va.gov

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------------------------------

Message: 5
Date: Fri, 05 May 2006 17:21:28 -0400
From: Andrea Hooper 
Subject: Re: [Histonet] counter-top tissue processors
To: John Paul 
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <47ec5a7c1d205.445b8998 <@t> med.cornell.edu>
Content-Type: text/plain; charset=us-ascii

In the past I used the Leica TP1020 and found it to be *very* unsatisfactory - in fact we returned it and got an 
automated Leica TP1050 which was a dream to work with. I suggest you get a full sized processor as in my 
experience they are far superior. If you have cost concerns get a refurbished one but buy from a reliable 
instrument company.

----- Original Message -----
From: John Paul 
Date: Friday, May 5, 2006 9:56 am
Subject: [Histonet] counter-top tissue processors

> Hi everyone
> We are looking to buy a counter-top (new or used) tissue 
> processor (paraffin) for a small research lab. I would appreciate 
> any recommendations from the group.
> Have a great day!!
> John
> 
> 
> ---------------------------------
> Yahoo! Mail goes everywhere you do. Get it on your phone.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 6
Date: Fri, 05 May 2006 17:28:37 -0400
From: Jeff Silverman 

Subject: [Histonet] Marker for invasive carcinoma
To: bliven.laura <@t> marshfieldclinic.org,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <000001c6708a$df34b910$6401a8c0 <@t> jeffrey028c8d9>
Content-Type: text/plain; charset=us-ascii

Laura, 

Finally, my research interest is finding some use in practice :-). In the
breast and in many other organs, the normal resting periductal, perilobular,
and interstitial fibroblasts express CD34. As carcinomatous invasion occurs,
resting CD34+ dendritic stromal fibroblasts transform into actin+
myofibroblasts so analysis of these two stromal markers are useful in
evaluating the presence or absence of carcinomatous invasion. Double stains
for cytokeratin and alpha smooth muscle actin have been used to demonstrate
the presence of invasion ie carcinoma cells surrounded by or amidst
myofibroblasts. These contractile cells are necessary to effect migration
through the connective tissue. The actin antibody will also detect the
presence or absence of myoepithelial cells in a given lesion. 

http://www.blackwell-synergy.com/links/doi/10.1046%2Fj.1365-2559.1996.d01-51
0.x

http://jcp.bmjjournals.com/cgi/content/full/56/4/271

This CD34 versus smooth muscle actin approach was recently reported useful
in evaluating invasive implants of peritoneal mesothelioma versus benign
endosalpingiosis in omentum and peritoneum. 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve
bstract&list_uids=16415795&query_hl=1&itool=pubmed_docsum>
&db=pubmed&dopt=Abstract&list_uids=16415795&query_hl=1&itool=pubmed_docsum

Hope this helps. 

Jeff Silverman HT HTL QIHC (ASCP)

Pathologists' Assistant- Pathology Supervisor

Southside Hospital

North Shore Long Island Jewish Health System

Bay Shore, New York USA

------------------------------

Message: 7
Date: Fri, 05 May 2006 17:34:14 -0400
From: Andrea Hooper 
Subject: Re: [Histonet] texture/quality of DAB staining
To: Ashley Haines 
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <48ca4e7d1ed65.445b8c96 <@t> med.cornell.edu>
Content-Type: text/plain; charset=us-ascii

Hi Ashley,

Your pic isn't on the site yet so I haven't been able to see precisely what you are talking about but I have a few 
suggestions to increase staining based on your protocol:

(1) I am assuming from your protocol and desire to counterstain with WG that you are doing cytospins of fresh 
cells????
(2) Did you try other fixations besides methanol? I find that methanol sometimes can reduce staining for certain 
antigens?
(3) Use 0.3% H202 in H20 for 10 minutes as 3% is harsh for cytospins or fresh tissue.
(4) Add another amplification step such as using a primary, biotinylated secondary and then Strep-HRP. Using a 
directly labeled primary will reduce your overall amplification.
(5) You could try Vector's ABC instead of Strep-HRP, your staining will be more intense though be careful for 
background
(6) Are you using DAB+? It is more robust than DAB
(7) Do primary overnight at 4 deg C and potentially increase primary concentration.

I will try to give more comments once I see the picture,
Andrea

----- Original Message -----
From: Ashley Haines 
Date: Friday, May 5, 2006 8:14 am
Subject: [Histonet] texture/quality of DAB staining
> 
> I am posting a picture called "fine DAB staining"
> (http://www.histonet.org/site_images_frame.asp). I am hoping for
> suggestions on how to improve the quality of the staining. In some 
> sensesit's a great result. You can see, at least under the scope 
> if not in the
> posted micrograph, lots of fine detail. There are many fine 
> projectionsfrom the cell surface which stain positive. 
> Unfortunately, my goal is to
> counterstain with a Wright Geimsa-like or Hema3 stain. The signal 
> I am
> getting will not be visible with that counterstain. Can you 
> suggest any
> ways to make my staining coarser and/or darker. Here's my basic 
> protocolfor the posted micrograph:
> 
> 
> MeOH fix cytospin, block with 3% H202 in MeOH 1hr at RT, wash, 
> block with
> 10% goat serum 1 hr, wash, incubate with biotinylated primary 1hr 
> at 37,
> wash, incubate with SA-HRP (1:1000) for 1 hr at RT, wash, detect with
> Vector's DAB substrate kit (with nickel enhancement) 20 min at RT, 
> wash in
> DI.
> 
> 

------------------------------

Message: 8
Date: Fri, 05 May 2006 17:39:12 -0400
From: Jeff Silverman 

Subject: [Histonet] Pancreas markers
To: Jackie.O'Connor <@t> abbott.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <000501c6708c$5959d670$6401a8c0 <@t> jeffrey028c8d9>
Content-Type: text/plain; charset=us-ascii

Pancreas specific markers?- no such thing but..

For ductal adenocarcinomas-MUC 1 is useful
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve
bstract&list_uids=14681945&query_hl=10&itool=pubmed_docsum>
&db=pubmed&dopt=Abstract&list_uids=14681945&query_hl=10&itool=pubmed_docsum

For colloid carcinomas- MUC 2 helps

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve
bstract&list_uids=12717243&query_hl=10&itool=pubmed_docsum>
&db=pubmed&dopt=Abstract&list_uids=12717243&query_hl=10&itool=pubmed_docsum

For islet cell neoplasia- chromogranin an or any of the possible hormones
(insulin, gastrin, glucagon etc)

Jeff Silverman HT HTL QIHC (ASCP)

Pathologists' Assistant- Pathology Supervisor

Southside Hospital

North Shore Long Island Jewish Health System

Bay Shore, New York USA

------------------------------

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End of Histonet Digest, Vol 30, Issue 8
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