[Histonet] Protocols for estimating the swelling of tissue?

[Kemlo Rogerson]

That's interesting. Is that in all fixatives? I could understand an aqueous
fixative initially causing such swelling as the water may reach the proteins
before the fixative; I also understand that acids are put into fixatives in
part to counteract the shrinking effects, but I would be surprised if an
alcoholic fixative caused any swelling initially at all.

How could it? As it removed water and caused the condensation of the protein
molecules how could that make the tissue swell?

If the tissue shrank or swelled then you think you could use water
displacement to measure the changes but I suppose that would only measures
the contraction due to increased cross linking of proteins as any water
removed would enter the vessel and therefore counteract any displacement as
the organ shrunk.

I don't think I've understood what I've just said. 

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

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-----Original Message-----
From: Charles Scouten [mailto:cwscouten <@t> myneurolab.com] 
Sent: Thursday, May 04, 2006 3:03 PM
To: Kemlo Rogerson; eboyden3 <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
Cc: Microscopy Today
Subject: RE: [Histonet] Protocols for estimating the swelling of tissue?

 If you are taking a whole small organ, you can measure its volume with
a Plethysmometer.  But I think volume of tissue is not what you want,
but a percentage of size change from normal.  Not easy to get.
Fixatives causes cells to swell on first exposure, but then shrink and
the whole organ or tissue shrinks.  There is an article coming out in
the May issue of Microscopy Today that addresses these issues, but does
not solve your problem.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, May 04, 2006 2:04 AM
To: 'eboyden3 <@t> gmail.com'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Protocols for estimating the swelling of tissue?

I thought, and I stand to be corrected, that most fixatives caused
shrinkage of tissue. I assumed that to be due to the fixation,
condensation, cross linking of proteins or the eradication of water
molecules. Hypertonic sucrose would also cause shrinkage too as it would
exert a negative osmotic pressure on the 'more dilute' tissue, wouldn't
it or have I got my pressures all wrong.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

One's action ought to come out of an achieved stillness: not to be a
mere rushing on. -- D.H. Lawrence 

This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its
contents: to do so is strictly prohibited and may be unlawful. Please
inform me that this message has gone astray before deleting it. Thank
you for your co-operation 

 


-----Original Message-----
From: Ed Boyden [mailto:eboyden3 <@t> gmail.com]
Sent: Thursday, May 04, 2006 12:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Protocols for estimating the swelling of tissue?

Many procedures (fixation, immersion in sucrose, etc.) cause swelling of
tissue, versus the intact, original tissue.  Does anyone know of
either:
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or  2. a protcol that would allow swelling of the tissue by a
predictable amount?

I would like to be able to compare samples done at different times, and
stored in different ways.

If not possible, I would like to be able to prepare new samples so that
their swelling will match my old estimates, as much as possible, or at
least that I can calculate the swelling of the new samples.

Thanks so much for your help!

Best,
Ed

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