[Histonet] Protocols for estimating the swelling of tissue?

[Charles Scouten]

 If you are taking a whole small organ, you can measure its volume with
a Plethysmometer.  But I think volume of tissue is not what you want,
but a percentage of size change from normal.  Not easy to get.
Fixatives causes cells to swell on first exposure, but then shrink and
the whole organ or tissue shrinks.  There is an article coming out in
the May issue of Microscopy Today that addresses these issues, but does
not solve your problem.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, May 04, 2006 2:04 AM
To: 'eboyden3 <@t> gmail.com'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Protocols for estimating the swelling of tissue?

I thought, and I stand to be corrected, that most fixatives caused
shrinkage of tissue. I assumed that to be due to the fixation,
condensation, cross linking of proteins or the eradication of water
molecules. Hypertonic sucrose would also cause shrinkage too as it would
exert a negative osmotic pressure on the 'more dilute' tissue, wouldn't
it or have I got my pressures all wrong.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

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-----Original Message-----
From: Ed Boyden [mailto:eboyden3 <@t> gmail.com]
Sent: Thursday, May 04, 2006 12:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Protocols for estimating the swelling of tissue?

Many procedures (fixation, immersion in sucrose, etc.) cause swelling of
tissue, versus the intact, original tissue.  Does anyone know of
either:
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or  2. a protcol that would allow swelling of the tissue by a
predictable amount?

I would like to be able to compare samples done at different times, and
stored in different ways.

If not possible, I would like to be able to prepare new samples so that
their swelling will match my old estimates, as much as possible, or at
least that I can calculate the swelling of the new samples.

Thanks so much for your help!

Best,
Ed

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