[Histonet] RE: Anyone use glass knifes anymore?

Wed May 3 14:39:19 CDT 2006

I use glass knives (triangles) that I make from 1/2 inch glass on an
ancient knife maker that I play heck keeping operating. I, too, am
frustrated by how hard it is to make decent knives.  I section plastic
embedded mouse brains at between 1 and 3 microns and do light
microscopy.  Even when my knives are working well, I can expect to use
two or three per mouse brain, in order to get the sections I want.  My
glass pieces are not the long strips of 1/4 inch pieces (although I have
used them), but shorter rectangles.  I think if you can get your knife
maker working well, it can make a difference.  I just had mine sent off
to Energy Beam Sciences, and they got it working, but then they shipped
it back to me and I really think that the shipper dropped it on the way,
because it has not been working well since.  I understand your
frustration, although I don't have an answer.  I wondered if the fact
that my glass is old would make a difference.  I know just enough
chemistry to remember that glass is a liquid that does change form as it
ages, and this glass was given to me and was old then.  Does anyone know
if that would affect it?
Gayle L. Brosnan-Watters, PhD
Assistant Professor
Dept of Psychology
Treasurer, Faculty for Undergraduate Neuroscience
226 Vincent Science Hall
Slippery Rock University
Slippery Rock, PA 16057
gayle.brosnanwatters <@t> sru.edu
724-738-2529 - office
724-738-4807 - fax

>  I make and use glass knifes for plastic sectioning
> at 3 microns. I usually have to go through a whole
> glass strip to get one good knife. Does anyone make
> them anymore and have the same problem?
> 
> 
> ~Stephanie Brusig
> 
> Weyerhaeuser Company
> Propagation of High Value Trees
> 32901 Weyerhaeuser Way S
> WTC-1B10
> Federal Way, WA 98001
> 253.924.6518
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
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> On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
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> Subject: Histonet Digest, Vol 30, Issue 3
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> Today's Topics:
> 
>    1. CONTROL BLOCKS NEEDED (Nymeyer, Heather)
>    2. Large sections (Patsy Ruegg)
>    3. Autostainer tubes  (Patti Loykasek)
>    4. NSH Active Members (Carrie Diamond)
>    5. Question (Nita Searcy)
>    6. Re: Question (Victoria Baker)
>    7. Re: Large sections (Gayle Callis)
>    8. RE: Question (Allen, Rhonda)
>    9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR))
>   10. Re: Autostainer tubes (Joanne Mauger)
>   11. Re: POC for Genetic Studies
> (histology <@t> gradymem.org)
>   12. antibodies to eIF2 alpha and PPAR gamma (Sarka
> Lhotak)
>   13. IHC on frozens (Sarka Lhotak)
>   14. Fwd: [Histonet] CD Abs (Linresearch <@t> aol.com)
>   15. gallocyanin stain for nissel  (Elizabeth
> Chlipala)
>   16. RANK, ER and PR in mouse (Randolph-Habecker,
> Julie)
>   17. NADH staining (Yak-Nam Wang)
>   18. Re: gallocyanine stain for Nissl (John
> Kiernan)
>   19. Inflamatory markers (CRAIG BARLOW)
>   20. Re- processing fatty tissues (Malam
> Jacqueline)
>   21. The end of an era (McGovern, Kevin)
>   22. Mycobacterium bovis (BCG) antibody (Martha
> Ward)
>   23. Glycerol cryoprotected tissue and cryostat
> sectioning
>       (Kathie A Berghorn)
>   24. RE: IHC on frozens (Guillermo Palao)
>   25. Save These Dates For The Pennsylvania State
> Histology	Meeting
>       (Pamela Marcum)
>   26. RE: Mycobacterium bovis (BCG) antibody
> (Elizabeth Chlipala)
> 
> 
>
----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 2 May 2006 10:03:23 -0700
> From: "Nymeyer, Heather"
> <Heather.Nymeyer <@t> interiorhealth.ca>
> Subject: [Histonet] CONTROL BLOCKS NEEDED
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 
>
<463911DE3F9F8D4AA3EC67632386BC62D1509E <@t> dc1serv78.interiorhealth.ca>
> Content-Type: text/plain;	charset="us-ascii"
> 
> We are in need of the following control blocks
> 
>             - Leprosy bacilli
> 
>             - Spirochete 
> 
>  
> 
> Your assistance is appreciated and thank you in
> advance.
> 
>  
> 
> Heather D. Nymeyer, RT, CEBT
> 
> Charge Technologist, Anatomic Pathology
> 
> Royal Inland Hospital,
> 
> Kamloops, BC.
> 
> 250-314-2664
> 
>  
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Tue, 2 May 2006 10:52:50 -0600
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: [Histonet] Large sections
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <200605021652.k42Gqoog014137 <@t> chip.viawest.net>
> Content-Type: text/plain;	charset="US-ASCII"
> 
> Can anyone suggest a resource for getting frozen
> sections of Elk Larynx?
>  
> One of my clients asks:
> "I study elk sound production. As one question we
> investigate the anatomical structure of the larynx.
> For this purpose I need cross sections of several
> larynges (approx. 15 larynges). Cross sections at
> three positions are necessary for each larynx,. The
> larynges are most likely calcified at varying
> degrees. I have stored the larynges frozen in saline
> solution."
>  
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net <http://www.ihctech.net/> 
>  
> 
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> 
> ------------------------------
> 
> Message: 3
> Date: Tue, 02 May 2006 10:31:31 -0700
> From: Patti Loykasek <ploykasek <@t> phenopath.com>
> Subject: [Histonet] Autostainer tubes
> To: histonet <histonet <@t> pathology.swmed.edu>
> Message-ID: <C07CE583.C181%ploykasek <@t> phenopath.com>
> Content-Type: text/plain; charset="US-ASCII"
> 
> Opps - didn't put in there a supplier other than
> Dako. We had a backorder problem with Dako on these.
> Actually turned out to be a glitch in their system,
> so all is well. Thanks for all of the good info,
> though. Histotechs are a nice bunch of people!
> 
> 
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
> 
> 
> 
> 
=== message truncated ===

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