[Histonet] Accidentally frozen NBF fixed tissue

[Nora Berghoff]

Hello all,
 
Someone in our lab accidentally froze 10% NBF fixed pancreas at -80
degree C.
They now want to get slides for H&E etc off that. Does anyone know
whether this tissue is still usable after being frozen? 
Obviously it is not good to freeze fixed tissue, but has anyone ever
done that and still gotten usable results? I would appreciate any input!

 
Thanks so much,
 
Nora Berghoff
 
 
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Nora Berghoff, med. vet.
Research Assistant
Gastrointestinal Laboratory
College of Veterinary Medicine
Texas A&M University
Phone: (979) 458 2293 (w)
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