[Histonet] RE: human-on-human IHC

[C.M. van der Loos]

   Dear Brett,

   A  couple  of  days  ago I wrote here about the Zenon rabbit-on-rabbit
   kit.  Well,  there  is  also  a human-on-human kit available from this
   vendor  (Molecular  Probes/Invitrogen). It is based on a labeled (some
   Alexa-fluorochromes, biotin, HRP) anti-human IgG Fab-fragment used for
   the  in  vitro  labeling  of  the  humanized  primary.  Basically, the
   technology behind is identical with the Dako ARKit.

   Good luck,

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Mon, 1 May 2006 11:50:30 -0400
   From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
   Subject: [Histonet] human-on-human IHC
   To: histonet <@t> lists.utsouthwestern.edu
   H'netter's
   I need advise on using human antibodies on human tissue. It will be an
   unlabeled human primary.
   i.e.  what  blocking  protocol  do  you recommend...any other specific
   caveats
   you can point out?
   Thanks,
   Brett
   Brett M. Connolly, Ph.D.
   Merck & Co., Inc.
   MRL, Imaging Research
   WP-44K
   PO Box 4
   West Point, PA 19486
   PH 215-652-2501
   fax. 215-993-6803
   e-mail. brett_connolly <@t> merck.com