[Histonet] Liver sections

[Rachael Emerson]

Hello. I need some advice about cutting adult mouse livers. The livers are
fixed in paraformaldehyde overnight, rinsed, stored in 70% EtOH, and then
processed into paraffin.  I usually cut at about 4um, but my sections look
like they are thicker...maybe 10um.  Everything else I cut looks fine, it¹s
just the liver.   Usually I face the block and let it sit on ice for 1-2
hours before cutting.  Sometimes if I am having trouble with shredding, I
will let it sit overnight on ice at 4°C.  Any advice or suggestions on my
thickness problem would be greatly appreciated.

Thank you
Rachael

-- 
Rachael L. Emerson 
Center for Pediatric Biomedical Research
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642