[Histonet] Re: marker for mouse vs. human

MVaughan4 <@t> ucok.edu MVaughan4 <@t> ucok.edu
Fri Mar 31 16:23:47 CST 2006

If you were using immunofluorescence I could tell you that Hoescht 
staining will demostrate punctate staining in AT-rich regions of the mouse 
nucleus, whereas human nuclei stain with a smoky appearance. Whether you 
could use this same distinction using paraffin sections I don't know. Also 
there are human-specific vimentin (DAKO) and Involucrin (BTI) antibodies 
available that supposedly will not stain mouse.
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034

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