[Histonet] DAB
Rene J Buesa
rjbuesa <@t> yahoo.com
Fri Mar 31 10:49:38 CST 2006
Hi Susan:
I always used nickel salts when I wanted to detect 2 different epitopes in the same section.
One I detected with regular DAB to get the "classical" permanent brown-reddish color. To detect the second epitope I used a DAB modification with nickel salts that will give an also permanent signal, but this time "blue-purplish" in a way that I could visualize both in the same section.
I prepared the second chromogen as follows:
a- DAB ---- 6 mg
b- PBS ---- 10 mL
c- 3% H2O2 --- 160 µL
d- 16.2% aq. solution of nickel sulfate hexahydrated --- 120 µL
Mix like the regular DAB and apply to the section an let act for 6 minutes.
I hope this will help you.
René J.
Susan.Ferrigon <@t> sanofi-aventis.com wrote:
Hi
Does anyone have a method or any experience on Nickel enhancement for DAB.
Any advice would be greatly appreciated.
Thanks Susan
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