[Histonet] paraffin tissue embedding and cutting
kemlo.rogerson <@t> waht.swest.nhs.uk
Fri Mar 31 01:25:31 CST 2006
Either xylene contamination or wax contaminated. I have noticed if you use
freshly melted wax then you get the bubbles, you may wish to put it in a
vacuum to lower SVP which ought to 'draw out' the bubbles. Sometimes you
find that the wax melts on the water bath as if its too hot; if the bath is
at the optimum temperature then look at xylene contamination or something
'wrong' with the wax.
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From: karen Cai [mailto:kcai <@t> prosci-inc.com]
Sent: Thursday, March 30, 2006 11:13 PM
To: HISTONET <@t> LISTS.UTSOUTHWESTERN.EDU
Subject: [Histonet] paraffin tissue embedding and cutting
I have some questions regarding embedding and cutting paraffin tissue
1. After embedding, I found there are a lot of bubbles inside the
paraffin tissue block (I put the mold at RT). Sometimes it has,
sometimes not. I don't know why. How to avoid this?
2. Sometimes I found if I put the paraffin tissue sections into
the 37C water bath, the paraffin will be diffused very quickly. Also the
color of the whole paraffin tissue block look strange: white, not clear
white. I am jus wondering whether the method of embedding tissue is
wrong, or something else?
Any kind of help will be very appreciated,
Thanks in advance,
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