[Histonet] paraffin tissue embedding and cutting

[Kemlo Rogerson]

Either xylene contamination or wax contaminated. I have noticed if you use
freshly melted wax then you get the bubbles, you may wish to put it in a
vacuum to lower SVP which ought to 'draw out' the bubbles. Sometimes you
find that the wax melts on the water bath as if its too hot; if the bath is
at the optimum temperature then look at xylene contamination or something
'wrong' with the wax.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

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-----Original Message-----
From: karen Cai [mailto:kcai <@t> prosci-inc.com] 
Sent: Thursday, March 30, 2006 11:13 PM
To: HISTONET <@t> LISTS.UTSOUTHWESTERN.EDU
Subject: [Histonet] paraffin tissue embedding and cutting 

Hello there,
I have some questions regarding embedding and cutting paraffin tissue
block:
 
1.	After embedding, I found there are a lot of bubbles inside the
paraffin tissue block (I put the mold at RT). Sometimes it has,
sometimes not.  I don't know why. How to avoid this?
2.	 Sometimes I found if I put the paraffin tissue sections into
the 37C water bath, the paraffin will be diffused very quickly. Also the
color of the whole paraffin tissue block look strange: white, not clear
white. I am jus wondering whether the method of embedding  tissue is
wrong, or  something else?
 
Any kind of help will be very appreciated,
 
Thanks in advance,
 
Best,
Karen
 
 
 

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