[Histonet] paraffin tissue embedding and cutting
kcai <@t> prosci-inc.com
Thu Mar 30 16:13:21 CST 2006
I have some questions regarding embedding and cutting paraffin tissue
1. After embedding, I found there are a lot of bubbles inside the
paraffin tissue block (I put the mold at RT). Sometimes it has,
sometimes not. I don=92t know why. How to avoid this?
2. Sometimes I found if I put the paraffin tissue sections into
the 37C water bath, the paraffin will be diffused very quickly. Also the
color of the whole paraffin tissue block look strange: white, not clear
white. I am jus wondering whether the method of embedding tissue is
wrong, or something else?
Any kind of help will be very appreciated,
Thanks in advance,
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