[Histonet] Frozen embedding of lungs

[Kim O'Sullivan]

Hi all,

Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs.

Any advice will be appreciated

Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Monash University
Melbourne
Australia